二磷酸盐和骨碎补总黄酮对诱导后成骨细胞影响的实验研究  

Experimental study on the effect of Diphosphonate and assemble flavone of drynaria rhizome on the induced osteoblast

作　　者：田坤[1] 尹文哲[1] 刘伟[2] 薛震[1] 赵金东[1] 

机构地区：[1]哈尔滨医科大学附属第二医院骨二科 [2]哈尔滨医科大学附属第二医院实验中心

出　　处：《中国骨质疏松杂志》2007年第8期559-563,共5页Chinese Journal of Osteoporosis

摘　　要：目的探索二磷酸盐和骨碎补总黄酮联合作用如何在分子水平发挥作用于成骨细胞,揭示其效果如何。方法使用诱导方法作用于骨髓基质干细胞,使之转化为成骨细胞;用RT-PCR检验成骨细胞;不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基,作用于成骨细胞,并分析生长曲线,分析细胞基因碱性磷酸酶（alkaline phosphatase ALP）和胶原酶Ⅰ（collagenase colⅠ）表达情况。结果骨髓基质细胞经过4周诱导可转化为表达colⅠ和ALP的成骨细胞,经过生长曲线分析,二磷酸盐和骨碎补总黄酮10^-4^-10-6mmol·L^-1时,对诱导后的成骨细胞有抑制作用;10^-8mmol·L^-1有促进作用;10^-10-10^-12mmol·L^-1不明显,其中10^-8mmol·L^-1作用最强;基因分析后,发现二磷酸盐和骨碎补总黄酮促进成骨细胞表达colⅠ和ALP,且两者合用效果大于单独用药。结论二磷酸盐和骨碎补总黄酮合用均可促进成骨细胞基因表达,且两者合用效果大于单独用药。Objective To study effects of diphosphonate and assemble tlavone of drynaria rhizome on the osteoblast at the level of molecule. Methods We isolated and cultured the MSCs, then induced MSCs （bone marrow stem cell MSCs） into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene （collagenase coli ）coli and （alkaline phosphatase ALP）ALP expression was detected. Results We can induce MSCs into osteoblasts which can express gene col Ⅰ and ALP. Through analysis growth curve, we demostrateed the diphosphonate or assemble tlavone of drynaria rhizome with 10^-4 - 10^-6 mmol·L^-1had the inhibition on the osteoblast; it with 10-s mmol·L^-1 had a promotion on the osteoblast; it with 10^-10 - 10^-12 mmol·L^-1 had a restraint on osteoblast. Conclusion Through gene analysis, we found the diphosphonate or assemble flavone of drynaria rhizome can have the promotion on the osteoblast. Especially, combined diphosphonate and assemble flavone of drynaria rhizome had more promotion on the osteoblast than diphosphonate or assemble flavone of drynaria rhizome lonely.

关 键 词：骨髓基质干细胞 成骨诱导 二磷酸盐 骨碎补总黄酮 

分 类 号：R285.5[医药卫生—中药学]