脓毒症后leptin对肠道功能的影响及对肝肾功能的保护作用  

Influence of leptin on intestinal function and its protective effect on hepatic and renal functions after sepsis

作　　者：颜光涛[1] 薛辉[1] 林季[1] 郝秀华[1] 张凯[1] 王录焕[1] 

机构地区：[1]解放军总医院基础医学研究所生化研究室,北京100853

出　　处：《中国病理生理杂志》2007年第8期1566-1569,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.39970717)

摘　　要：目的:研究脓毒症对肝、肾功能及肠道相关酶活性的影响,探讨瘦素(leptin)在急性炎症反应中的作用。方法:建立小鼠盲肠结扎致脓毒症模型,采用96孔分光光度法检测血清丙氨酸氨基转移酶(ALT)、尿酸(UA)和肠组织匀浆液中髓过氧化物酶(MPO)、谷胱甘肽-S转移酶(GST)、黄嘌呤氧化酶(XOD)、超氧化物歧化酶(SOD)等,同时以HE染色方法观察肠道组织病理学改变。结果:脓毒症后6 h,血清UA水平[(521.92±91.86)μmol/L]明显高于假手术组[(330.12±94.15)μmol/L],而12 h ALT[(83.55±40.44)U/L]和UA[(474.03±75.22)μmol/L]均高于假手术组[(66.23±16.80)U/L]和[(320.95±99.14)μmol/L]。采用腹腔内注射leptin(0.1 mg/kg)和吲哚美辛(2 mg/kg),12 h时ALT和UA水平均明显低于脓毒症组(P<0.05)。此外,伴有肠道组织MPO、GST、XOD和SOD活性的改变。结论:在脓毒症过程中,微量leptin体外注射可以发挥改善和稳定肝肾功能的明显作用,其机制可能同其影响肠道多种与自由基、巯基和嘌呤代谢相关酶的功能有关,而且吲哚美辛在一定程度上有类似的效应,但所用剂量明显高于leptin水平。AIM： To detect the effect of sepsis on hepatic, renal functions and corresponding enzymes in intestine of mice, and to explore the role of leptin in acute inflammation. METHODS ： A mice model of sepsis was made by cecum ligation and perforation, and 96 - well spectrophotometry was used to detecte the levels of alanine aminotransferase （ALT） , uric acid （UA） and activities of myeloperoxidase （ MPO）, glutathin - S - transferase （GST）, xanthine oxidase （ XOD）, superoxide dismutase （SOD） in serum and intestinal homogenized fluids, respectively. Hematoxylin - eosin staining was used simultaneously to check the histopathologic changes of intestine. RESULTS： Compared with sham group （330. 12 μmol/L ±94. 15 μmol/L）, serum UA level （521.92 μmol/L ±91.86 μmol/L） at 6 h after sepsis was significantly higher. 12 h after sepsis, both serum ALT （83.55 U/L ±40.44 U/L） and UA （474. 03 μmol/L ±75.22 μmol/L） were significantly higher than those in sham group （66. 23 U/L ± 16. 80 U/L and 320. 95 μmol/L ± 99. 14 μmol/L, respectively）. 12 h after leptin injection （0. 1 mg/kg, ip） or indomethacin injection （2 mg/kg, ip）, the serum ALT and UA levels significantly decreased （vs sepsis group, P 〈0.05）. Moreover, the activities of MPO, GST, XOD and SOD in intestine were changed at different degrees. CONCLUSION ： During the process of sepsis, trace dose of leptin injected peritoneally significantly improves and stabilizes the hepatic and renal functions. The mechanisms may be related with those intestinal enzymes associated with metabolism of free radicals, mercapto group and purine. Indomethacin exerts a similar role as leptin though at much higher dose.

关 键 词：瘦素 脓毒症 肠 过氧化物酶 肝 肾 

分 类 号：R392.32[医药卫生—免疫学]