类先天性马蹄足动物模型胚胎病理初步研究及病因学意义  

Primary Research and Etiological Implication of Congenital Clubfoot-like Model at Pathological and Molecule Level

作　　者：贾艳丽[1] 杜世新[2] 刘戈飞[3] 

机构地区：[1]汕头大学医学院第一附属医院矫形外科,广东汕头市515041 [2]汕头大学医学院第一附属医院骨科,广东汕头市515041 [3]汕头大学医学院细胞生物实验室,广东汕头市515031

出　　处：《中国康复理论与实践》2007年第8期733-735,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：广东省卫生厅医学科研基金资助项目(No.A2005445);广东省自然科学基金资助(No.5300584);广东省科技计划项目(No.2006B35901008)

摘　　要：目的分析在肢体发育不同时段综合多基因相互作用条件下最终导致先天性马蹄内翻足(CCF)发生的原因。方法77只大白鼠从怀孕第10天起,将维甲酸石蜡油混悬液经胃管单次注入,建立CCF动物模型;应用光镜、电镜及分子生物学方法检测胎鼠后肢芽(后肢)、胸腰段脊髓。结果动物模型的CCF发病率为61.8%,距骨发育停滞,距骨、跟骨间重叠不良和跟骨内翻,后肢芽及脊髓前角细胞均有凋亡现象。结论CCF在胚胎发育期即有畸形,且程度随生长发育而逐步加重,后肢芽及脊髓前角细胞在胚胎早期即出现凋亡现象。Objective To explore the possible correlations under the interaction of multi-genes action at different stages and analyze the primary histophotomacrographic changes of hind buds tissue in congenital clubfoot and the pathodynamic developmental procedure. Methods Seventy-seven female Wistar rats were administered with retinoic acid on the 10th day after pregnancy. And the hindlimb, buds and spinal cord were detected through transmission electron microscopic and molecular biological experiments. Results There was clubfoot-like deformity in 61.8 % of the experimental animals. Persistence of the embryonic position of the talus and tibia in fetuses was observed. Poor overlapping between talus and calcaneus was seen. Cell apoptosis at the anterior corner of spinal cord and hind buds were seen. Conclusion Congenital clubfoot deformities appear at early stage and exaggerate along with developmental procedure.

关 键 词：足畸形 先天性 维甲酸 凋亡 

分 类 号：R682.1[医药卫生—骨科学]