性别决定与分化相关基因在大鼠肝再生中的表达模式和作用分析  

EXPRESSION PATTERNS AND FUNCTION ANALYSIS OF THE GENES RELATED WITH SEX DETERMINATION AND DIFFERENTIATION DURING RAT LIVER REGENERATION

作　　者：李鹏鸽[1] 阴棉棉[1] 赵利峰[2] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]河南师范大学省部共建细胞分化调控重点实验室,新乡453007

出　　处：《解剖学报》2007年第4期446-451,共6页Acta Anatomica Sinica

基　　金：河南师范大学大学生科研基金资助项目(20060365)

摘　　要：目的在基因转录水平了解调控性别决定与分化基因在肝再生中的作用。方法查阅相关论文和NCBI、GENMAPP、KEGG、BIOCARTA、RGD等网站获得调控性别决定与分化基因,用Rat Genome 230 2.0芯片分析它们在大鼠肝再生中表达变化和作用。结果肝再生启动[部分肝切除(PH)后0.5-4h]、G0/G1过渡(PH后4-6h)、细胞增殖(PH后6-66h)、细胞再分化和组织结构功能重建(PH后72-168h)等4个阶段起始表达的基因数为41、61、8和3;总表达的基因数为41、25、57和41。表明肝再生相关基因主要在肝再生启动阶段开始表达,在不同阶段发挥作用。它们表达的相似性分为均上调、上调占优势、均下调、下调占优势、上调和下调次数相近等5类,涉及22、9、15、9和7个基因,共表达上调231次、下调146次,表明肝再生中多数基因表达加强,少数基因表达降低。它们表达的时间相关性分为15组,表明肝再生中细胞生理生化活动具有阶段性。它们的表达模式分为20类,表明肝再生中细胞生理生化活动多样和复杂。结论雄性性别决定、分化和雌性性别分化相关基因主要在肝再生晚早期和前期表达增强,雌性性别决定相关基因主要在肝再生前期表达增强,与肝再生密切相关。Objective To study the function of the genes regulating sex determination and differentiation during liver regeneration at transcriptional level. Methods The genes regulating sex determination and differentiation were obtained by referring to the theses and collecting the data of databases at NCBI, GENMAPP, KEGG, BIOCARTA and RGD, and their function and expression changes in rat liver regeneration were analysized by the Rat Genome 230 2.0 array. Results The initial and total expressed gene numbers in the starting phase of liver regeneration [ half to four hours after partial hepatectomy （PH）], G0/G1 transition （4 to 6 hours after PH）, cell proliferation 6 to 66 hours after PH）, cell differentiation and tissue structural function reconstruction （72 to 160 hours after PH） were 41,6,18,3 and 41,25,57,41 respectively, which showed that the related genes were mainly triggered in the starting phase, and worked in different phases. Their expression similarity was classified into 5 groups：only up-, predominantly up-, only down- predominantly down-, up-/down-regulation, involving 22,9,15,9 and 7 genes respectively, and ihe total frequencies of their up- and down-regulation expressions were 231 and 146 respectively, demonstrating that the expression of the major genes was increased, and the minority decreased. Their expression time relevance was classified into 15 groups, showing that the cellular physiological and biochemical activities were phase related during liver regeneration. The gene expression patterns were classified into 20 types, indicating the diversity and complexity of the cellular physiological and biochemical activity. Conclusion The genes regulating male determination, male and female differentiation are enhanced mainly in the late early phase and prophase of liver regeneration, and the genes regulating female determination are enhanced mainly in the prophase. The function of the genes is closely related to liver regeneration.

关 键 词：部分肝切除 性别决定 性别分化 肝再生相关基因 Rat Genome 230 2.0芯片 

分 类 号：Q254[生物学—细胞生物学]