p21活化激酶2在爪蟾卵母细胞的细胞质分裂过程中的作用  

FUNCTION OF p21-ACTIVATED KINASE 2 IN XENOPUS OOCYTE CYTOKINESIS

作　　者：程大也[1] 梁彬[1] 李丰[1] 

机构地区：[1]中国医科大学细胞生物学卫生部重点实验室

出　　处：《解剖学报》2007年第4期476-480,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30370736;30570966);教育部博士点基金资助项目(20050159023);教育部回国人员科研启动基金资助项目(2004527)

摘　　要：目的研究p21活化激酶2(PAK2)在爪蟾卵母细胞的细胞质分裂中的作用。方法以爪蟾卵母细胞为模型,利用特异性抑制PAK2活性的PAK2-N端(PAK2-NT)片段,显微注射爪蟾卵母细胞。荧光显微镜下比较PAK2-NT mRNA注射组和对照组卵母细胞生发泡破裂的发生。激光扫描共焦显微镜下,时间延迟摄影法观察两组卵母细胞的细胞质分裂过程中肌动蛋白和纺锤体的变化。结果PAK2-NT mRNA注射组卵母细胞与对照组卵母细胞生发泡破裂发生相似,但PAK2-NT mRNA注射组卵母细胞未见细胞质分裂。结论PAK2可能参与爪蟾卵母细胞的细胞质分裂过程。Objective Study the function of p21-activated kinase 2 in Xenopus oocyte cytokinesis. Methods Xenopus oocyte was used as a model, and MPF activity and PAK2 phosphorylation status were observed during oocyte maturation. The specific inhibitor of PAK2 activity, PAK2-NT （PAK2-N-terminal） was used to microinject into Xenopus oocyte. Under fluorescent microscope germinal vesicle breakdown was observed during cytokinesis. To further observe the changes of F-actin and spindle, laser scanning confocal microscopy with time-lapse method was employed. Results Occurrences of germinal vesicle breakdown in control oocytes was similar to those oocytes injected with PAK2-NT mRNA, but no cytokinesis was observed in oocytes injected with PAK2-NT mRNA. Conclusion These results indicated that PAK2 might involved in Xenopus oocytes cytokinesis.

关 键 词：P21活化激酶 卵母细胞 细胞质分裂 显微注射 激光扫描共焦显微镜 爪蟾 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]