转化生长因子-β1及其信号传导分子Smad2与Smad4在羊驼睾丸的表达  

EXPRESSION OF TRANSFORMING GROWTH FACTOR-β1 AND ITS SIGNAL TRANSDUCER SMAD2 AND SMAD4 IN ALPACA TESTIS

作　　者：董常生[1] 闫永平[2] 贺俊平[1] 赫晓燕[1] 任玉红[1] 白瑞[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]河北保定职业技术学院畜牧兽医系,保定071051

出　　处：《解剖学报》2007年第4期481-485,共5页Acta Anatomica Sinica

基　　金：引进国际先进农业科学技术计划(948计划)(202096)(2003-Z86)提供资助

摘　　要：目的研究转化生长因子-β1(TGF-β1)、Smad2、Smad4在羊驼睾丸的表达与定位,探索TGF-β1在羊驼睾丸发育及精子发生中的作用。方法以24月龄的羊驼睾丸为研究对象,采用Western blotting技术及免疫组织化学SABC法对TGF-β1、Smad2、Smad4在羊驼睾丸的表达进行研究。结果Western blotting结果显示,羊驼睾丸组织粗蛋白提取物中存在分子量约为25kD5、2kD、62kD,与多克隆兔抗TGF-β1、Smad2、Smad4抗体发生免疫阳性反应的蛋白条带;免疫组织化学结果显示,TGF-β1、Smad2、Smad4在24月龄羊驼睾丸的间质细胞、支持细胞和各级生精细胞均有不同程度的表达。结论TGF-β1通过Smad2与Smad4传导途径参与性成熟羊驼睾丸组织的发育与精子发生的调控。Objective To study the expression and localization of TGF-β1 and its signal transducer Smad2 and Smad4 which play important roles during testicular development and spermatogenesis in alpaca testis. Methods The whole testes were obtained from alpacas aged 24 months （ n = 3 ） at the Scientific Research Base of Shanxi Agriculture University. The protein expressions of TGF-β1,smad2 ,smad4 in alpaca testes were examined by Western blotting and SABC. Results The distributions of TGF-β1 ,Smad2 and Smad4 in the testes of alpacas aged 24 months were demonstrated. Conclusion Our findings show that TGF-β1, Smad2 and Smad4 are involved in the regulation of spermatogenesis, and provide direct evidences for the moleculer mechnism of TGF-β1 action in the alpaca testis during spermatogenesis.

关 键 词：睾丸 转化生长因子-Β1 SMAD2 SMAD4 免疫印迹法 免疫组织化学 羊驼 

分 类 号：Q959.837[生物学—动物学]