刀豆蛋白A诱导大鼠小肠黏膜微血管内皮细胞分泌IFN-γ的研究  

ConA INDUCES INTESTINAL MUCOUS MICROVASCULAR ENDOTHELIAL CELLS TO SECRETE IFN-γ

作　　者：尹龙[1] 胡格[1] 索占伟[1] 段慧琴[1] 张涛[2] 穆祥[1] 

机构地区：[1]北京农学院动物科学和技术系,北京102206 [2]中国农业大学动物医学院,北京100094

出　　处：《解剖学报》2007年第4期486-488,共3页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30500368);北京市自然科学基金A类重点项目(6061001);北京市教委"学术创新团队"(5090245);北京市科技新星项目(2006A26)

摘　　要：目的探讨刀豆蛋白A(ConA)能否诱导肠黏膜微血管内皮细胞分泌IFN-γ。方法采用双抗夹心ELISA法检测细胞培养上清液中的IFN-γ的含量。结果ConA能极显著地激活大鼠肠黏膜微血管内皮细胞分泌IFN-γ,5.0mg/L ConA的激活作用有时间依赖性,但10.0mg/L ConA激活作用未表明有时间依赖性;5.0mg/L ConA组和10.0mg/L ConA组间无显著性差异。结论ConA能激活大鼠小肠黏膜微血管内皮细胞分泌IFN-γ。Objective To study whether ConA could induce microvascular endothelial cells to secrete IFN-γ. Methods IFN-γ was tested by double antibody sandwich ELISA. Results The microvascular endothelial cells of the intestinal mucous could be remarkably activated by ConA to secret IFN-γ, and the activations of 5.0mg/L ConA was time-dependent, but the 10.0 mg/L ConA was not so;There was not obviously different between the group of 5.0mg/L ConA and that of 10.0mg/L ConA. Conclusion ConA could induce microvascular endothelial cells of the rat intestinal mucosa to secrete IFN-T.

关 键 词：Γ-干扰素 刀豆蛋白A 肠粘膜微血管内皮细胞 ELISA法 大鼠 

分 类 号：Q233[生物学—细胞生物学]