大肠埃希菌新的氨基糖苷类修饰酶基因研究  

Detection of Novel Resistance Genes Encoded Aminoglycoside-modifying Enzymes from Clinical Isolates of Escherichia coli

作　　者：李智山[1] 周乐翔[1] 赵建忠[1] 杨燕[1] 邓三季[1] 邹玖明[1] 

机构地区：[1]华中科技大学同济医学院附属襄樊医院,湖北襄樊441021

出　　处：《中华医院感染学杂志》2007年第8期914-916,共3页Chinese Journal of Nosocomiology

摘　　要：目的了解大肠埃希菌(ECO)新的氨基糖苷类修饰酶基因存在状况。方法用聚合酶链反应(PCR)法对34株分离自患者的ECO进行aac(6′)-Ⅰb、aac(6′)-Ⅱ、aac(3)-Ⅰ、aac(3)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ等6种氨基糖苷类修饰酶基因检测研究。结果34株ECO中氨基糖苷类修饰酶基因aac(6′)-Ⅰb阳性8株(23.5%)、aac(3)-Ⅱ阳性27株(39.4%)、ant(3″)-Ⅰ阳性3株(8.8%),其余基因均阴性。结论氨基糖苷类高耐药性与氨基糖苷类修饰酶基因高检出率(85.3%)有关;在同一所医院的ECO中同时检出aac(6′)-Ⅰb和aac(6′)-Ⅰb-cr两种亚型,为国内外首次报道。OBJECTⅠVE To detect novel resistance genes encoded aminoglycoside-modifyi：ng enzymes from clinical isolates of Escherichia coll. METHODS All isolates were subjected to polymerase chain reaction（PCR） assays targeting aminoglycoside-modifying enzymes genes including aac（6＇）-Ⅰ b,aac（6＇）-Ⅱ , aac（3）-Ⅰ , aac（3）-Ⅱ , ant （3＂）- Ⅰ and ant（2＂）- Ⅰ . RESULTS Ⅰn thirty-four strains of E. coli, aac（6＇）- Ⅰ b were detected in 8 （23.5%）, aac （3）- Ⅱ in 27 （39.4 ~A ）, ant （3＂）- Ⅰ in 3 （8.8 %）, and others were negative. CONCLUSⅠONS This study showed that high resistance to aminoglycosides was related to high positive rate （85.3%） of aminoglycoside-modifying enzymes genes in E. coli~ and it was also first reported that both aac（6＇）- Ⅰ b and aac（6＇）- Ⅰ b-cr were detected in clinical isolates of E. coli only from a hospital.

关 键 词：大肠埃希菌 氨基糖苷类修饰酶基因 聚合酶链反应 

分 类 号：R378.21[医药卫生—病原生物学]