B7-H1协同刺激淋巴细胞增殖并诱导产生抑制性T细胞  

B7-H1 enhances Ag-specific lymphocytes proliferation and induces immunosuppressive T cells

作　　者：辛利军[1] 焦志军[1] 丁庆[1] 路丽明[1] 周芸[1] 周光炎[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025

出　　处：《中国免疫学杂志》2007年第8期680-683,687,共5页Chinese Journal of Immunology

基　　金：国家863项目子课题(2003AA205009);上海市科委项目(03JC14085);上海市教委青年项目(04BC30)资助

摘　　要：目的:探讨B7-H1分子在抗原诱导的免疫反应中的作用。方法:在可溶性抗原PPD诱导淋巴细胞增殖体系中,加入中和抗体检测B7-H1的协同刺激作用。转染表达B7-H1的ECV304细胞,观测其对PPD诱导的淋巴细胞增殖、细胞因子分泌及淋巴细胞亚群比例的影响。将刺激后的淋巴细胞过继至自体或同种异体增殖体系中,检测其功能。结果:抗B7-H1中和抗体可降低PPD诱导的淋巴细胞增殖反应和细胞因子分泌;转染B7-H1的ECV304细胞可协同刺激抗原诱导的淋巴细胞增殖,促进细胞因子IL-10的表达,并改变T细胞亚群分布,增加CD4+T细胞亚群比例;自体或同种异体过继实验显示,B7-H1刺激后的淋巴细胞具有显著的免疫抑制功能。结论:B7-H1分子可协同刺激淋巴细胞增殖并诱导产生具有免疫抑制功能的T细胞亚群。To investigate the function of costimulatory factor B7-H1 in antigen-specific immune response. Methods ：The effect of B7-H1 in soluble antigen（PPD）-induced lymphocyte proliferation was detected by using anti-B7-H1 neutralizing antibody. Then, B7-Hl-transfected ECV304 cell line and mock-transfected control were used to stimulate PPD-induced lymphocytes, respectively, which were examined for proliferation, cytokines secretion, cytokine mRNA level and changes of T cell subsets. The function of T cell stimulated with the B7-Hl-transfected ECV304 cell line was also inspected through autoor aUo-lymphocyte adoptive transfering assay. Results：Antigen-specific lymphocyte proliferation and cytokine secretion were inhibited by anti-B7-H1 neutralizing antibody. Compared with mock-transfected control, the B7-Hl-transfected ECV304 cells enhanced the proliferation of PPD-stimulated lymphocytes, promoted the secretion of IL-10 and expression of its mRNA. Besides, the ratio of T cell subsets was also changed owing to the enlargement of CD4^＋ T cell populations. In adoptive transfering assay, lymphocytes stimulated with the B7-Hl-transfected ECV304 cells markedly inhibited autoor aUo-lymphocytes proliferation. Conclusion： Costimulatory factor B7-H1 could enhance antigen-specific lymphocytes proliferation and induce some T cell subset with immunosuppressive activity.

关 键 词：B7-H1 淋巴细胞增殖 IL-10 免疫抑制 

分 类 号：R392.12[医药卫生—免疫学]