负载乳腺癌细胞冻融抗原的DC对CTL体外特异性杀伤活性的影响  

Study on DC loaded with freeze-thrawing antigen from breast cancer cells to the specific cytotoxicity of CTL in vitro

作　　者：刘运江[1] 张建立[1] 单保恩[1] 

机构地区：[1]河北医科大学第四医院,石家庄050011

出　　处：《中国免疫学杂志》2007年第8期693-696,共4页Chinese Journal of Immunology

基　　金：河北省科技攻关课题(05276101D-66);河北省强势特色学科科研项目

摘　　要：目的:研究乳腺癌患者腋下淋巴结单个核细胞来源的DC,经自体肿瘤细胞冻融抗原刺激后,对CTL体外特异性杀伤活性的影响。方法:以细胞因子分别诱导、培养乳腺癌腋下淋巴结单个核细胞中的DC及TDLNC,用自体癌细胞冻融抗原刺激DC,并和TDLNC共培养,诱导成为肿瘤抗原特异性CTL;检测特异性CTL对自体乳腺癌细胞及MCF-7细胞的体外杀伤活性。结果:DC-Ag-TDLNC、DC-TDLNC和TDLNC对自体乳腺癌细胞的杀伤率分别为67.64%、31.25%和26.36%,P<0.001;三种TDLNC细胞对MCF-7细胞的杀伤率无明显差异(P>0.05)。结论:乳腺癌患者腋下引流淋巴结中的单个核细胞在细胞因子的诱导、活化及自体肿瘤抗原刺激下,可分化为成熟DC,DC可促进TDLNC增殖、分化为特异性CTL,后者对自体肿瘤细胞有较高的杀伤活性。To study the influence of the DCs from mononuclear cells in axillary draining lymph nodes of patients with breast cancer to the cytotoxicity of the antigenic specific CTLs stimulated by freeze-thrawing antigen from breast cancer cells in vitro. Methods：The mononuclear cells were isolated from axillary draining lymph nodes, and were cultured with cytokines to induce DCs and tumor draining lymph node cells （TDLNCs） respectively. DCs stimulated by the auto-breast cancer freeze-thrawing antigen （DCs-Ag）. TDLNCs were co-cultured with DCs-Ag to derive tumor antigenic specific CTLs（DC-Ag-TDLNC）. Then the cytotoxicty of specific CTLs to auto-breast cancer cells and MCF-7 cells was detected. Results：The cytotoxicity of DC-Ag-TDLNC, DC-TDLNC and TDLNC to the auto-breast cancer cells was 67. 64% .31.25% and 26. 36% respectively, P 〈0. 001. There was no significant difference in cytotoxicity of the three group CTLs to the MCF-7 cells, P 〉 0. 05. Conclusion ： Typical DCs can be induced from the mononu- clear cells from axillary draining lymph nodes after stimulated with cytokines （rhGM-CSF, rhIL-4 and TNF-ot）. The DCs possess stronger antigen presentation and can obviously increase the killing activity of tumor antigen specific CTLs to auto-breast cancer cells.

关 键 词：乳腺癌 腋窝引流淋巴结 树突状细胞 细胞因子 特异性细胞毒T淋巴细胞 

分 类 号：R392.11[医药卫生—免疫学]