尿路致病性大肠杆菌Ⅰ型菌毛的FimH蛋白与DNA免疫效果比较  

Comparison among the immune effects of DNA-or protein-FimH of UPEC type 1 pilus

作　　者：尹晓琳[1] 石新丽[2] 魏林[1] 马翠卿[1] 王秀荣[1] 冯惠东[1] 

机构地区：[1]河北医科大学,石家庄050017 [2]石家庄市卫生学校,石家庄050200

出　　处：《中国免疫学杂志》2007年第8期752-754,757,共4页Chinese Journal of Immunology

基　　金：河北省卫生厅基金资助项目(基金号:02076)

摘　　要：目的:用尿路致病性大肠杆菌(UPEC)Ⅰ型菌毛FimH蛋白的DNA和蛋白免疫小鼠,以观察不同组别的细胞免疫、体液免疫和黏膜免疫的效果。方法:将fimH质粒、fimC质粒,FimH、FimC蛋白经多种组合免疫小鼠,ELISA法检测外周血中抗FimH蛋白的特异性IgG和膀胱中的SIgA,流式细胞仪检测脾脏中CD3、CD4、CD8三种T细胞亚群的变化。结果:DNA免疫后血清中可检测出特异性IgG,但效价较低,可检测出较低效价的SIgA,脾脏中CD4+T细胞百分含量较高,引起较强的细胞免疫。蛋白免疫后血清特异性IgG较高,发生较强的体液免疫,未测出SIgA。结论:FimH蛋白的DNA疫苗引起细胞免疫、体液免疫和黏膜免疫,蛋白疫苗只能诱导体液免疫,FimC蛋白能增强FimH蛋白的免疫原性。To observe cellular, humoral and mucosal immune responses induced by DNAor protein-based of FimH of UPEC type 1. Methods： After mice were immunized respectively with recombinant plasmid pcDNA3.1/fimH or pcDNA3.1/fimC, and the combinant FimH and FimC protein, the anti-FimH protein IgG of sera and SIgA in bladders were detected by ELISA. The lymphocyte phenotypes of CD3, CD4 and CD8 were analyzed by FCM. Results： The titers of IgG in sera and SIgA in the bladders were all low in the group immunized by recombinant FimH plamid, but the percentage of CD4^4 T cells in spleen was high, which revealed that recombinant FimH plamid was able to trigger better cellular immune response. The titers of IgG were very high in the group immunized by FimH protein, which suggested that the FimH protein was able to trigger better humoral immune response, but SIgA in the bladders was not detectable. Conduslon：The DNA for FimH can induce humoral, mucosal and cellular immune response. FimH protein can only induce humoral immune response. FimC protein is able to enhance the immunogenicity of FimH protein.

关 键 词：尿路致病性大肠杆菌 Ⅰ型菌毛 fimH基因 蛋白疫苗 核酸疫苗 

分 类 号：R378.21[医药卫生—病原生物学]