人膀胱组织中P2X受体亚型表达的实验研究  

Expression of P2X receptor subtypes in human bladder

作　　者：王伟[1] 马琳[1] 许金荣[2] 高红[1] 

机构地区：[1]中国医科大学附属盛京医院小儿外科,辽宁沈阳110004 [2]中国医科大学附属盛京医院内科

出　　处：《中国医科大学学报》2007年第4期450-452,共3页Journal of China Medical University

基　　金：辽宁省教育厅高等学校科研基金资助项目(05L495)

摘　　要：目的:检测正常儿膀胱颈部和顶部的P2X受体亚型mRNA的表达情况,并探讨其临床意义。方法:提取正常儿膀胱颈部和顶部的总mRNA,以β-actin为内参照,采用RT-PCR方法检测P2X受体各亚型mRNA在膀胱中的表达情况。结果:膀胱颈部和顶部均发现了P2X受体的7种亚型mRNA表达。颈部及顶部受体亚型的表达水平顺序分别为:P2X7>P2X1>P2X4>P2X3>P2X2>P2X5>P2X6和P2X4>P2X2>P2X1>P2X3>P2X7>P2X5>P2X6。结论:正常儿膀胱内有P2X受体的7种亚型mRNA表达,提示P2X受体亚型对膀胱感觉和运动功能的调节作用具有深入研究的价值。Objective： To evaluate the mRNA expressions of P2X receptor subtypes in the bladders of normal newborns and to explore their clinical significance. Methods： Total mRNA was extracted from the neck and dome of the bladders of normal newborns. The mRNA expressions of P2X receptor subtypes in the bladder tissues were detected by reverse transcription polymerase chain reaction,using β-actin as internal control. Results： The mRNA expressions of 7 P2X receptor subtypes were found at both bladder neck and bladder dome of normal newborns. The expression levels of 7 P2X receptor subtypes decreased from P2X7 to P2X1 to P2X4 to P2X3 to P2X2 to P2X5 to P2X6 at bladder neck and from P2X4 to P2X2 to P2X1 to P2X3 to P2X7 to P2X5 to P2X6 at bladder dome,respectively. Conclusion： Seven P2X receptor subtypes are expressed in the bladders of normal newborns. It is worthy of further studying the role of the P2X receptor subtypes in the sensory and motor functions of bladder.

关 键 词：P2X受体 膀胱 逆转录聚合酶链式反应 

分 类 号：R334.3[医药卫生—人体生理学]