bDNA和NASBA法定量检测HIV-1病毒载量的比较研究  

A comparative study on testing methods for HIV-1 viral load by bDNA and NASBA

作　　者：郭秋艳[1] 黄晓婕[2] 代丽丽[2] 汪习成[2] 魏飞力[2] 计云霞[2] 石英[2] 夏伟[2] 郭彩萍[2] 吴亚松[2] 张彤[2] 陈德喜[2] 吴昊[2] 

机构地区：[1]首都医科大学附属北京佑安医院中心实验室,北京100069 [2]首都医科大学附属北京佑安医院感染科

出　　处：《中国病原生物学杂志》2007年第5期326-328,共3页Journal of Pathogen Biology

基　　金：北京市科委科技计划重大项目(No.D0906003040591)。

摘　　要：目的比较分支DNA杂交实验(branched DNA,bDNA)和核酸序列扩增实验(Nucleic acid sequence-based am-plification,NASBA)两种方法检测人类免疫缺陷病毒1型病毒(HIV-1)病毒载量间的一致性。方法对25例HIV感染者/艾滋病(Acquired immune deficiency syndrome,AIDS)患者血浆标本同时用bDNA法和NASBA法检测HIV-1病毒载量,并用流式细胞术检测患者外周血CD4+、CD8+T淋巴细胞。结果bDNA法及NASBA法测得HIV-1病毒载量平均值分别为(4.398±0.580)log拷贝数/ml和(4.488±0.602)log拷贝数/ml,差异无统计学意义(t=1.210,P>0.05);两种方法检测出的病毒载量呈显著直线相关(r=0.8004,P<0.001),且与患者的CD4+T细胞数及CD4+/CD8+比值均呈显著直线负相关。结论bDNA法和NASBA法检测HIV-1病毒载量具有高度一致性,在实际工作中均可选用。Objective To investigate the consistency between two different testing methods for HIV-1 viral load by branched DNA （bDNA） and Nucleic acid sequence-based amplification （NASBA）. Methods All blood samples from 25 cases with HIV-1 infection and AIDS patients were tested for HIV-1 viral load by bDNA and NASBA and peripheral blood CD4^＋ , CD8^＋ T lymphocytes by flow cytometry. Results The average HIV viral load detected by bDNA and NASBA was （4. 398±0. 580）log copies/ml and （4. 488±0. 602）log copies/ml respectively, and the difference was no startistically significant between the two groups（t= 1,210,P〉0.05）. There was a significant linear correlation between the two methods（r=0. 8004,P〈0. 001）. Viral load of the two methods had a significant negative linear correlation with the CD4＋ T cell counts and CD4^＋/CD8^＋ ratios. Conclusion There is a high degree coincidence between the two methods （bDNA and NASBA） for detecting HIV viral load and they are both available in the actual work.

关 键 词：HIV病毒载量 NASBA法 bDNA法 

分 类 号：R373.9[医药卫生—病原生物学]