多房棘球绦虫重组BCG-Em14-3-3疫苗免疫小鼠后脾T细胞亚群的动态观察  

Dynamic observation on subsets of spleen T cells in mice by immunization with recombinant BCG-Em14-3-3 vaccine of Echinococcus multilocularis

作　　者：李文桂[1] 王鸿[1] 朱佑明[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《中国病原生物学杂志》2007年第5期347-349,共3页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.30671835;30500423和30200239);教育部重点项目(No.205131);重庆市教委科研基金项目(No.KJ050313);重庆市科委科研基金项目(No.03-43-8);重庆市卫生局科研基金项目(No.03-2-099)

摘　　要：目的动态观察多房棘球绦虫重组BCG-Em14-3-3疫苗免疫小鼠后脾T细胞亚群的变化。方法将疫苗采用鼻腔内接种方法免疫BALB/c鼠,在免疫后0、2、4、6、8、10、12、14、16和18周各剖杀4只小鼠,取脾,分离脾细胞,流式细胞仪检测脾CD4+和CD8+T淋巴细胞亚群的百分比,同时设PBS对照。结果疫苗接种组小鼠脾CD4+和CD8+T细胞亚群分别在免疫后2～12周和2～18周开始升高,分别在免疫后6和10周达最高水平,百分比分别为32.0%±1.6%和8.4%±0.8%。结论CD4+和CD8+T细胞亚群在多房棘球绦虫重组BCG-Em14-3-3疫苗诱导的小鼠抗Em原头节攻击感染的保护性免疫机制中起关键作用。Objective To dynamically observe changes on subsets of spleen T cells in mice by immunization with recombinant BCG-Em14-3-3 vaccine of Echinococcus multilocularis（Em）. Methods BALB/c mice were intranasally vaccinated by the vaccine and killed to get spleen on 0,2,4,6,8,10,12,14,16 and 18 weeks of immunization respectively, spleen cells were separated to measure subsets of CD4^＋ and CD8^＋ T cells by FACsort, PBS served as control. Results In the groups of immunization, CD4^＋ and CD8^＋ subsets increased obviously on 2-12 weeks and 2-18 weeks respectively, reached the highest level on 6 and 10 weeks respectively. Conclusion CD4^＋ and CD8^＋ subsets may play an important role in protection induced in mice by rBCG-Em14-3-3 vaccine against challenge with Em protoscoleces.

关 键 词：多房棘球绦虫 重组BCG-EM14-3-3疫苗 脾T细胞亚群 

分 类 号：R383.33[医药卫生—医学寄生虫学]