红花HPLC的指纹图谱研究  

Fingerprint of Carthamus Tinctorius L. by HPLC

作　　者：谢国祥[1] 邱明丰[1] 张汉杰[1] 翟秀华[1] 王誉洁[1] 贾伟[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《中成药》2007年第8期1093-1097,共5页Chinese Traditional Patent Medicine

基　　金：上海市科委中药现代化专项(06DZ19707)

摘　　要：目的:利用高效液相色谱建立红花的色谱指纹图谱。方法:采用反相C18柱,乙腈-0.5磷酸水溶液梯度洗脱;检测波长275nm;流速1.0mL/min进行试验。结果:从10个省出产的红花获得了34个色谱共有峰,基本特征一致。羟基红花黄色素A为指标成分,新疆红花的含量为最高。结论:该法为中药样品的鉴定提供了较全面的信息,红花样品指纹图谱及指标成分含量测定可用于全面控制红花药材的质量。AIM： To establish the chromatographic fingerprint of Carthamus Tinctorius L. by high performance liquid chromatography （HPLC）. METHODS ： The chromatographic fingerprint of Carthamus Tinctorius L. was established by HPLC with a RP-C18 column, acetonitrile-water （0.5% phosphoric acid） gradient elution, a DAD diode array detector at 275 nm and the flow rate of 1.0 mL/min, column temperature of 25℃ o RESULTS ： 34 copeaks of Carthamus Tiuctorius L. were gained from 10 producing provinces. Its basic features were rough consistent each other. Hydrosafflor yellow A, was selected as marker, among samples the content of Xinjiang was the highest in the all samples. CONCLUSION： This method provides useful information for identification of traditional Chinese medicines, the fingerprints of samples of Carthamus Tinctorius L. can be used to control the quality combined with content determination.

关 键 词：红花 高效液相色谱 羟基红花黄色素A 色谱指纹图谱 

分 类 号：R284.1[医药卫生—中药学]