肠胃清对KB-A-1细胞P-糖蛋白ATP酶活性及MDR1基因转录水平的影响  

Effect of Changweiqing on P-glucoprotein ATPase activity and MDR1 genetic transcription in KB-A-1 cells

作　　者：许建华[1] 范忠泽[1] 孙珏[1] 朱美华[1] 石晓兰[1] 韩建宏[1] 张勇[1] 李朝衡[1] 

机构地区：[1]上海中医药大学附属普陀医院,上海200062

出　　处：《中成药》2007年第8期1119-1122,共4页Chinese Traditional Patent Medicine

摘　　要：目的:探讨肠胃清(黄芪、党参、白术等)逆转肿瘤多药耐药的作用机制。方法:制作肠胃清药物血清,观察药物血清对多药耐药细胞KB-A-1P-糖蛋白ATP酶活性及MDR1基因转录水平的影响。结果:肠胃清药物血清对P-gp的ATP酶活性具有抑制作用,并呈剂量依赖性;2.5和5药物血清对MDR1基因转录水平的抑制作用分别达到了58和100。结论:肠胃清逆转肿瘤耐药与其抑制P-gp的酶活性、降低MDR1mRNA表达有关。AIM： To explore the mechanism of reversal action of Changweiqing oral liquid（ Radix Astragali, Radix Codonopsis, Rhizoma Atracty lodis macroce phalae,etc. ） on malignant cell multidrug resistance. METHODS： Blood srum containing Changweiqing was made and its effect was observed on P-glucoprotein ATPase activity and MDR1 genetic transcription in KB-A-1 cells. RESULTS： Blood serum containing Changweiqing could inhibit Pglncoprotein ATPase activity with dose dependence ;2.5% and 5% of drug serum could decrease the level of MDR1 genetic transcription by 58% and 100% respectively. CONCLUSION： The reversal action of Changweiqing oral liquid has related with its depressant effect on P-glueoprotein ATPase activity and MDR1 genetic transcription.

关 键 词：肠胃清 多药耐药 P-糖蛋白 KB-A-1细胞 

分 类 号：R285.6[医药卫生—中药学]