RP-HPLC测定连蒲双清片中咖啡酸、槲皮素和盐酸小檗碱  

Determination of caffeic acid,quercetin and berberine hydrochloride in Lianpu Shuangqing Tablets by RP-HPLC

作　　者：谭明娜[1] 于清峰[1] 肖莹[1] 倪坤仪[1] 

机构地区：[1]中国药科大学分析化学教研室

出　　处：《中成药》2007年第8期1161-1164,共4页Chinese Traditional Patent Medicine

摘　　要：目的：建立连蒲双清片（蒲公英,盐酸小檗碱）中咖啡酸、槲皮素和盐酸小檗碱的HPLC测定方法。方法：采用C18柱（AichromBond-1,4.6mm×250mm,5μm）,甲醇-磷酸-三乙胺-水为流动相,检测波长321nm,流速为1mL/min。结果：咖啡酸、槲皮素和盐酸小檗碱的线性范围分别为2.00-30.0μg/mL（r=0.9997）,2.40~16.0μg/mL（r=0.9991）,15.0-90.0μg/mL（r=0.9993）,平均加样回收率（n=5）分别为97.8、100.6和100.4;RSD分别为2.7、1.5和1.4。结论：该方法准确,重复性好,专属性高,适用于连蒲双清片的质量控制。AIM： To establish the method for determining caffeic acid, quercetin and berberine hydrochloride in Lianpu Shuangqing Tablets（Herba Taraxaci, berberine hydrochloride） by RP-HPLC. METHODS ： The column was an AichromBond-1 C18 column（4.6 mm ×250 mm, 5 μm）. The mobile phase was made up of CH3OH- H3PO4-C6H15N-H2O. The ultraviolet detection was set at 321 nm. The flow rate was 1 mL/min. RESULTS： The linear ranges of caffeic acid, quercetin and berberine hydrochloride were 2.00-30.0 μg/mL（ r = 0. 999 7）, 2.40- 16.0 μg/mL（r =0. 999 1 ）, 15.0-90.0 μg/mL（ r = 0. 999 3 ）, respectively. The average recoverise（ n = 5 ） of caffeic acid, quercetin and berberine hydrochloride were 97.8%, 100.6% and 100.4% ; RSD were 2.7%, 1.5% and 1.4%, respectively. CONCLUSION： The method is accurate, reproducible and highly selective, thus suitable for quality control of Lianpu Shuangqing Tablets.

关 键 词：RP-HPLC 梯度洗脱 咖啡酸 槲皮素 盐酸小檗碱 

分 类 号：R927.2[医药卫生—药学]