运用血清药理学方法研究生脉散对豚鼠离体工作心脏的作用  

Effects of Shenmai San on Hemodynamics in Isolated Working Guinea Pigs Hearts by Serum Pharmacology

作　　者：刘红[1] 艾民仙[1] 阳辉[1] 

机构地区：[1]湖北民族学院医学院,湖北恩施445000

出　　处：《时珍国医国药》2007年第8期1815-1816,共2页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省卫生厅科研基金项目(No.JXIB120)

摘　　要：目的观察家兔灌胃给予生脉散后,其含药血清对豚鼠离体工作心脏血流动力学的影响。方法家兔按分组灌胃分别给予生脉散5,10 ml.kg-1,2次/d,连续3 d,末次给药后60 min取血,分离血清。制备豚鼠离体工作心脏,观察含药血清对心率(HR)、动脉血压(AP)、左室收缩压(LVSP)、左室舒张期末压(LVEDP)、左室内压最大上升及下降速率(±dp/dtmax)的影响。结果含药血清在给药后不同时间具有减慢HR,降低AP,LVEDP,增高LVSP和±dp/dtm ax的作用,且呈明显的剂量依赖关系(P<0.01)。结论含药血清具有正性肌力和负性频率作用。Objective To observe the effects of Shenmai San on hemodynamics in isolated working guinea pigs hearts. Methods The experiment was accomplished by serum pharmacology. Different ig dosages（5,10 ml·　kg^-1） were administered to rabbits twice a day for three consecutive days. The blood was taken at 60 min after the last administration and the serum was separated. The separated serum was added to the isolated working guinea pigs hearts. The effects of Shenmai San on heart rate （HR） , arterial blood pressure （AP） ,left ventricular systolic pressure （LVSP） ,left ventricular end - diastolic pressure （LVEDP） , and ±dp/dtmax were observed. Results The herbal Shenmai San serum slowed HR and reduced AP, LVEDP, increase LVSP and ±dp/dtmax （P 〈 0. 01 ） in a dose -dependent manner. Conclusion The results suggest that the herbal Shenmai San serum may cause positive inotropic effect and reduce rhythum of the heart and blood pressure in isolated working heart in a dose - dependent manner.

关 键 词：生脉散 血清药理学 离体工作心脏 

分 类 号：R285.5[医药卫生—中药学]