高致病性禽流感病毒性肺炎小鼠模型血清细胞因子的水平  

Significance and Assaying of Serum Interleukin-1β,Interleukin-6,Interleukin-8 and Tumor Necrosis Factor-α in Highly Pathogenic Avian Influenza Viral Pneumonia in Mice

作　　者：赵宏霞[1] 夏咸柱[1] 鲁继荣[2] 

机构地区：[1]中国人民解放军军事医学科学院军事兽医研究所 [2]吉林大学第一医院儿科,长春130061

出　　处：《实用儿科临床杂志》2007年第16期1217-1219,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(30471295)

摘　　要：目的探讨细胞因子IL-1β、IL-6、IL-8和肿瘤坏死因子-α(TNF-α)在高致病性禽流感病毒性肺炎(HPAIVP)中的作用。方法昆明小鼠60只随机分为实验组和对照组,每组30只。高致病性禽流感病毒滴鼻制备HPAIVP小鼠模型,采用双抗体夹心酶联免疫吸附法(ELISA),对攻毒后不同时间点实验和对照组小鼠血清IL-1β、IL-6、IL-8和TNF-α水平进行检测。结果实验组血清IL-1β、IL-6、和TNF-α水平明显高于对照组,差异均有显著性(Pa<0.05)。结论细胞因子IL-1β、IL-6和TNF-α参与高致病性禽流感病毒肺炎的发病过程。Objective To study the interrelation between highly pathogenic avian influenza viral pneumonia （HPAIVP） and cellular factors interlenkin - 1β （ IL - 1β）, interleukin - 6 （ IL - 6 ）, interleukin - 8 （ IL - 8 ） and tumor necrosis factor - α （ TNF - α） in HPAIVP. Methods Sixty Kunming mice were divided into 2 groups randomly ： experiment group and control group, each group consisted of 30 mice. The highly pathogenic avian influenza virus was used to establish HPAIDP models. The expressions of serum IL - 1β, IL - 6, IL - 8 and TNF - α in experiment group of different moment and control group were detected by enzyme linked immunoeerbent assay（ELISA）. Result The levels of serum IL - 1β, IL - 6 and TNF - α in experiment group were significantly higher than those in control group （ Pa 〈 0.01 ）. Conclusion The cellular factors IL - 1β, IL - 6 and TNF - α may participate in the whole process of HPAIV infection and their serum levels are positively correlated With the seriousness of the disease.

关 键 词：小鼠 禽流感病毒 高致病性 肺炎 白细胞介素1Β 白细胞介素6 白细胞介素8 肿瘤坏死因子-α 

分 类 号：R725.6[医药卫生—儿科]