非特异性角膜炎症反应中PDTC对LPS诱导LFA-1 mRNA表达的影响  

Influence of PDTC on expression of LFA-1 mRNA induced with LPS in mouse non-specific keratitis reaction

作　　者：武广恒[1] 张家颖[1] 杨丽红[2] 周乐[3] 

机构地区：[1]吉林大学基础医学院生物化学与分子生物学实验中心,吉林长春130021 [2]天津眼科医院白内障科,天津300020 [3]吉林大学

出　　处：《吉林大学学报（医学版）》2007年第4期612-615,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(39870313)

摘　　要：目的:探讨淋巴细胞功能相关抗原-1(LFA-1)的 mRNA表达与非特异性角膜炎症反应的相关性及核因子NF-κB抑制剂对LFA-1 mRNA表达的阻断作用。方法:建立BALB/C鼠角膜缝线及联合结膜下药物注射的动物模型,通过逆转录聚合酶链式反应(RT-PCR),对单纯缝线组、缝线联合脂多糖(LPS)组和缝线联合LPS及吡咯啉烷二硫代氨基甲酸盐(PDTC)组术后1、3、7和14d不同时相点角膜LFA-1 mRNA的转录水平进行检测。结果:术后第1、3、7d时,角膜缝线联合LPS处理组LFA-1 mRNA的表达明显高于单纯缝线组(P<0.05);14d时,角膜缝线联合LPS处理组与单纯缝线组比较差异无显著性(P>0.05)。而1和7d时缝线联合LPS和PDTC组LFA-1 mRNA的表达明显高于缝线联合LPS组;3和14d时缝线联合LPS和PDTC组LFA-1 mRNA的表达明显低于缝线联合LPS组(P<0.05)。结论:联合结膜下注射LPS可在术后诱导LFA-1 mRNA的高水平表达,而核因子NF-κB抑制剂PDTC可抑制角膜LFA-1 mRNA的表达,表明PDTC对LFA-1 mRNA表达具有阻断调控作用。Objective To analyze the relationship between leukocyte function-associated antigen-1 （LFA-1） mRNA expression and corneal inflammation and discuss the inhibition reaction of nuclear factor-κB （NF-κB） inhibitor on LFA-1 mRNA expression. Methods The corneal suture or combined with subconjunctival injection models were constructed in BALB/C mice. The corneas of each group were excised 1, 3, 7 and 14 d after operation and LFA-1 mRNA expressions were investigated by reverse transcription polymerase chain reaction （RT-PCR）. Results At 1, 3 and 7 d after operation, the expressions of corneal LFA-1 mRNA in corneal suture combined with lipopolysaccharide （LPS） （corneal suture＋LPs） subconjunctival injection group were higher than those in corneal suture group （P〈0.05） ; at 14 d after operation, there was no significant difference of the expression of corneal LFA-1 mRNA between two groups （P〉0.05）. At 1 and 7 d after operation, compared with corneal suture＋ LPS group , the expression of corneal LFA-1 mRNA increased, but it decreased at 3 and 14 d after operation in the group of corneal suture combined with LPS and pyrrolidine dithiocarbamate （PDTC） （P 〈 0.05 ）. Conclusion A high expression of LFA-1 mRNA can be induced by the corneal stutre combined with LPS subconjunctival injection. The inhibitor of NF-κB （PDTC） can inhibit the expression of corneal LFA-1 mRNA. The expression of LFA-1 mRNA can be blocked by PDTC.

关 键 词：淋巴细胞功能相关抗原-1 NF-KAPPA B 角膜炎 结膜下注射 吡咯啉烷二硫代氨基甲酸盐 脂多糖类 

分 类 号：R722.2[医药卫生—儿科] R-332[医药卫生—临床医学]