血清CK-MB活力假性增高原因分析及临床意义  

To analyse the cause of the false increased in serum CK-MB activity and its clinical significance

作　　者：吴凤丽[1] 马晓光[1] 孙玉珍[1] 冯静文 张丽霞[2] 

机构地区：[1]沈阳二四二医院检验科,辽宁沈阳110034 [2]中国医科大学附属第一医院检验科

出　　处：《中国实验诊断学》2007年第8期1041-1043,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨某些非心源性疾病患者血清肌酸激酶同工酶(CK-MB)活性升高的原因及与疾病的关系。方法采用日立7180型全自动生化分析仪测试CK及CK-MB,检出CK-MB异常升高者,且CK-MB/CK超过30%者,进一步用CK同工酶琼脂糖凝胶电泳鉴定,并结合病情进行分析。结果42例非心肌梗死患者血清标本中(包括3例溶血标本),总CK活力相对较低,癌症患者CK-MB假阳性比例较大,其中CK-MB占CK总活力在30%-49%有24例,大于50%的有18例。42例经CK同工酶电泳证实,CK-MB活性实际为正常,但出现脑型肌酸激酶同工酶(CK-BB)或异常CK同工酶带。结论应用酶免疫抑制法测定非心肌梗死患者CK-MB时,如果CK-MB超过CK30%以上,可能是血清中CK-BB、巨CK、腺苷酸激酶的存在,影响CK-MB的假性升高。Objective To study the cause of the false increased serum MB isoenzyme of ereatine ldnase（CK-MB） activity and its relationship with diseases.Methods Activities of total CK and CK-MB were determined by automatic biochemistry analyzer.Patients with increased CK-MB activity and CK-MB/CK ratio over 30% were selected to do the further agarose gel eletrophoresis of CK isoenzyme analysis and the results were reviewed according to the clinical diagnosis. Results Total CK activity was low in 42 serum specimen （ 3 haemolytic samples） with non-myocardial infarction, but in the patients with tumor, the pseudo-positive ratio of the CK-MB enzymatic activity was the highest, there were 24 cases, in which CK-MB activity accounted for 30% - 49 % enzymatic activity of CK, and 18 cases accounted for more than 50% .The CK-MB activity in all 42 cases was normal and BB isoenzyme of creatine kinase（CK- BB） or some other abnormal CK isoenzyme appeared. Conclusion Serum specimen of non-myocardial infarction patients were detected by enzyme linked immunonhibition, when over 30% of total CK is CK-MB,It may be caused by the CK-BB,macro-CK, AK in serum.

关 键 词：心肌型肌酸激酶 脑型肌酸激酶同工酶 巨肌酸激酶 免疫抑制法 

分 类 号：R446.1[医药卫生—诊断学]