荧光法研究DNA与蒽相互作用机理  

Mechanism of interaction between DNA and anthrancene

作　　者：李璐[1] 任娇蓉[2] 郜洪文[1] 潘碌亭[1] 

机构地区：[1]同济大学污染防治与资源化国家重点实验室,上海200092 [2]华东师范大学资源与环境科学学院,上海200062

出　　处：《环境污染与防治》2007年第8期592-594,共3页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.20477030);上海市重点基础研究项目(No.04JC14072)

摘　　要：利用荧光猝灭光谱和紫外-可见吸收光谱研究了体外DNA对蒽的荧光猝灭机理。结果表明,DNA的紫外-可见光谱没有发生红移现象,通过计算分析不同温度下的荧光猝灭常数和热力学参数,结合两种光谱分析可知DNA对蒽的荧光猝灭属于动态猝灭机理。Fluorescence spectra and UV spectra for anthrancene solutions with DNA at increasing concentration were employed for the study of the interaction between DNA and anthrancene at 25 ℃ ,35℃and 45 ℃, DNA exhibited a powerful effect of quenching anthrancene＇s fluorescence. The fluorescence quenching data were treated to fit both the Stern-Volmer equation and the Lineweaver-Burk equation, The data showing the dynamic quenching effect of DNA have suggested that the interaction was caused by molecular collisions rather than chemical reaction. 　

关 键 词：荧光猝灭 蒽 DNA 热力学参数 

分 类 号：X503[环境科学与工程—环境工程]