Triton X-100敏化鱼精蛋白共振能量转移荧光猝灭法测定尿中8-羟基脱氧鸟苷  

Determination of 8-Hydroxy-2'-Deoxyguanosine in Urine with Protamine Using Resonance Energy Transfer Fluorescence Quenching by Sensitizing Effect of Triton X-100

作　　者：米贤文[1] 王永生[1] 薛金花[1] 欧阳运富[1] 杨胜园[1] 王英[1] 

机构地区：[1]南华大学公共卫生学院

出　　处：《光谱学与光谱分析》2007年第8期1570-1573,共4页Spectroscopy and Spectral Analysis

基　　金：湖南省自然科学基金项目(03JJY3030)资助

摘　　要：研究了8-羟基脱氧鸟苷与鱼精蛋白之间的共振能量转移的最佳条件,建立了鱼精蛋白共振能量转移荧光猝灭法测定8-羟基脱氧鸟苷的新方法。在301 nm,pH 8.2的Tris-HCl缓冲溶液中,鱼精蛋白的荧光猝灭强度与8-羟基脱氧鸟苷的浓度成正比,工作曲线的线性范围为0.50-7.1μg·mL^-1,r=0.9967,方法的检出限为0.18μg·mL^-1,RSD=6.2%,平均加标回收率为104%（n=6）。 ct-DNA浓度的新试剂,与ct-DNA的作用有嵌入和静电吸引两种方式。The optimum conditions for the resonance energy transfer between 8-hydroxy-2＇-deoxyguanosine（8-OHdG） and protamine were studied, and a novel method for the determination of 8-OHdG has been developed based on the resonance energy transfer fluorescence quenching effect of protarnine. The fluorescence quenching intensity of Ps at 301 nm was proportional to the concentration of 8-OHdG. The linear range of the calibration curve was 0.50-7.1 vμg·mL^-1 in the pH 8.2 Tris-HC1 buffer solution with the detection limits of 0.18 μg·mL^-1. The relative standard deviation was 6.2% and the average recovery was 104% （n=6）. The proposed method is simple and sensitive, and has succeeded in analyzing the spiked urine sample with satisfactory results.

关 键 词：8-羟基脱氧鸟苷 鱼精蛋白 荧光猝灭 共振能量转移 

分 类 号：O657.3[理学—分析化学]