色谱光谱法测试超临界压力下穿心莲内酯的结晶规律  

Test of the Effect of Different Supercritical Pressure on Andrographolide Crystallization with Chromatography and Spectrum

作　　者：张文成[1] 张兴元[2] 潘见[1] 

机构地区：[1]合肥工业大学农产品生物化工教育部工程研究中心,安徽合肥230009 [2]中国科学技术大学高分子科学与工程系,安徽合肥230026

出　　处：《光谱学与光谱分析》2007年第8期1657-1660,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(29976008);教育部科技创新工程重大项目培育基金项目(704027)资助

摘　　要：以30%和95%的穿心莲内酯为实验原料,采用超临界CO2萃取结晶法考察了不同压力下穿心莲内酯在结晶板上的分布规律,同时也考察了晶体的晶型和红外光谱的变化规律。研究证实:高效液相色谱分析,不同压力下穿心莲内酯在结晶板上都按纯度梯度结晶分布;X射线衍射分析,压力越高,晶体越趋于向比较单一的晶面上择优生长;红外光谱分析,压力的变化,并没有引起穿心莲内酯化学结构的改变。With the new technique of supercritical carbon dioxide extraction and crystallization, the experimental materials of 30% and 95% andrographolide were used to investigate the effect of different pressure on crystal distribution on crystallization board, what＇s more, the effect of X-ray diffraction and infrared ray spectrum on crystal was tested. The results showed that andrographolide was crystallized on crystallization board in purity gradient with high performance liquid chromatography; the high- er the pressure, the higher the crystallization ratio. In addition, the crystal was inclining to directional growth; the chemical structure of andrographolide was not changed with the variety of pressure with infrared ray spectrum analysis.

关 键 词：超临界CO2 萃取结晶 穿心莲内酯 高效液相色谱 X射线衍射 红外光谱 

分 类 号：TQ02[化学工程] O79[理学—晶体学]