共表达O型口蹄疫病毒3C、P1-2A基因和猪白细胞介素18基因的重组鸡痘病毒免疫原性研究  

Immunogenicity of Recombinant Fowl-pox Vaccines Coexpressing Foot-and-Mouth Disease Virus 3C、P1-2A Gene and Porcine Interleukin 18

作　　者：刘慧娟[1] 金宁一[2] 马鸣潇[1] 金明兰[1] 沈国顺[1] 霍晓伟[1] 鲁会军[2] 金扩世[1] 李旭[1] 计越[1] 

机构地区：[1]中国人民解放军军事医学科学院军事兽医研究所全军基因工程重点实验室,长春130062 [2]吉林大学畜牧兽医学院,长春130062

出　　处：《中国生物工程杂志》2007年第8期25-28,共4页China Biotechnology

基　　金：国家"863"计划资助项目(2001AA213071);吉林省科技发展计划资助项目(20040202-1)

摘　　要：目的:对筛选到的一株共表达O型口蹄疫病毒3C基因、P1-2A基因和猪白细胞介素18基因的重组鸡痘病毒rFPV-3C-P1-2A-IL-18进行免疫原性研究。方法:分别用重组鸡痘病毒rFPV-3C-P1-2A-IL-18、rFPV-P1-2A-3C和对照组282E4株FPV、PBS对小鼠进行免疫接种,并检测各免疫组的T淋巴细胞亚类数量、CTL杀伤活性及抗体效价。结果:重组鸡痘病毒疫苗组免疫小鼠T淋巴细胞亚类数量,CTL杀伤活性和抗体效价均显著高于对照组,且重组鸡痘病毒rFPV-3C-P1-2A-IL-18的小鼠T淋巴细胞亚类数量和CTL特异性杀伤活性与rFPV-P1-2A-3C相比,差异显著。结论:为开发新型FMDV疫苗奠定了实验免疫基础。Objective：Evaluate the immunogenicity of an recombinant fowl-pox virus rFPV-3C-P1-2A-IL-18. Methods：Mice were inoculated with the recombinant FPV （ rFPV ） rFPV -3C -P1 -2A -IL-18, rFPV -P1 -2A -3C vaccines,282E4 FPV and PBS was set as the control groups. The FMDV specific antibody, number of T subtype, cytotoxicity of special CTL were detected. Results ： Compared with other control groups, the mice inoculated with rFPV-3 C - P1-2 A-IL-18, rFPV -P1-2 A-3 C vaccines were induced higher anti -FMDV antibody titer, alarger number of T subtype, and higher cytotoxicity of special CTL. Compared with rFPV-P1-2A-3C vaccine, rFPV-3C-P1-2A-IL -18 can induce larger number of T subtype and higher cytotoxicity of special CTL. Conclusion：h is evident that the recombinant fowl-pox virus rFPV-3C- P1-2A-IL-18 show excellent immunogenicity in mice, thus provide valuable support for further development of FMDV genetic engineering vaccines.

关 键 词：口蹄疫病毒 重组鸡痘病毒 免疫原性 

分 类 号：Q939.91[生物学—微生物学]