一种葡聚糖芯片的再生方法、表征及其应用  

A Method for Dextran Chip Regeneration, Characterization and Application

作　　者：李雪玲[1] 方志俊[2] 黄明辉[2] 曹慧敏[1] 赵建龙[1] 杨梦苏[1] 

机构地区：[1]中国科学院上海微系统与信息技术研究所,上海200050 [2]香港城市大学深圳研究院,深圳518057

出　　处：《中国生物工程杂志》2007年第8期59-62,共4页China Biotechnology

基　　金：国家"973"计划资助项目(2005CB724305);上海重点基础研究资助项目(04JC14081)

摘　　要：表面等离子体共振(surface plasmon resonance,SPR)生物传感器,作为一种适时快捷,无需标记的生物分子相互作用研究工具,已广泛应用于生物化学分析与研究。羧甲基化葡聚糖修饰的CM5传感芯片是Biacore系列仪器应用最为普遍的核心部件,目前CM5芯片主要从法玛西亚公司购买,价格昂贵,且一旦共价交联的受体分子失活,就不能重复利用。阐述了一种简便、低成本、用于SPR生物传感器的葡聚糖修饰金膜芯片的再生方法及其表征和应用。用此方法再生的芯片能被循环伏安法和原子力显微镜很好地表征,并成功地用于抗前列腺特异性抗原(prostate-specific antigen,PSA)固定和PSA检测,同时测定了PSA与其抗体之间的动力学和亲和常数。As a real-time, fast and label-free instrument for biomolecular interactions, surface plasmon resonance biosensor has been widely used in biomedical analysis and research. Carboxymethylated dextran modified CM5 sensor chip is the core of the Biacore instrument. CM5 sensor chips are now mostly purchased from Pharmacia Biosensor AB （Uppsala Sweden） , which are expensive and once the covalent immobilized receptor molecules have lost bioactivity, it cannot be reused. An easy, cost- effective method for regeneration, characterization and preparation of carboxymethylated dextran modified （CMD） sensor chips for SPR biosensor was described. The prepared CMD surface was well characterized by cyclic voltammetry and atomic force microscopy and successfully used for the detection of prostate-specific antigen in solution and the measurement of the constants of affinity and kinetics of the binding of PSA with PSA antibody.

关 键 词：SPR生物传感器 羧甲基化葡聚糖修饰表面 传感芯片再生 前列腺特异性抗原检测 动力学和亲和力 

分 类 号：Q6-3[生物学—生物物理学]