大鼠动脉粥样硬化形成过程中TAFI水平变化及作用机制  

TAFI level and its mechanism in the process of atherosclerosis in rats

作　　者：刘利妍[1] 刘向群[1] 陈效安[1] 陈焕芹[1] 

机构地区：[1]山东大学齐鲁医院保健科,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第8期787-791,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技发展计划项目(2006GG2202045)

摘　　要：目的:探讨在大鼠动脉粥样硬化(AS)形成过程中凝血酶活化的纤维蛋白溶解抑制剂(TAFI)活性水平变化及作用机制。方法:将40只健康的雄性Wistar大鼠随机分为正常对照组、高脂组、高脂维生素D3组和内皮损伤组,每组10只。分别以普通饲料、高脂饲料、高脂饲料+维生素D3负荷、高脂饲料+维生素D3负荷+内皮球囊损伤处理4个月,复制大鼠动脉粥样硬化形成过程中三个阶段的模型,即高脂血症、纤维增生性动脉硬化、较成熟的AS斑块病变。模型建造前,自颈动脉取血,模型建造成功后,自下腔静脉取血,用全自动生化仪测定血浆总胆固醇(TC)、总甘油三脂(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C),发色底物法测定血浆TAFI的活性,分析TAFI活性以及血脂指标在造模前、后不同组间的变化。结果:①成功复制了大鼠动脉粥样硬化发展过程中高脂血症期、纤维增生性动脉硬化期、较成熟的斑块期的模型;②高脂组、高脂维生素D3组和内皮损伤组大鼠的TC、TG、LDL-C水平和TAFI活性明显高于正常对照组(P<0.01),且有递增趋势,以内膜损伤组升高最为明显;三组模型鼠的HDL-C水平明显低于正常对照组(P<0.01或P<0.05),且有递减趋势,以内膜损伤组降低最为明显;③血浆TAFI活性与TC和TG水平呈正相关。结论:AS过程中,TAFI水平呈逐渐增高趋势,提示AS的病变程度与血浆TAFI水平密切相关。Objective： To investigate the level of TAFI and its mechanism on atherosclerosis. Methods： Forty healthy male Wistar rats were randomly divided into 4 groups （n = 10）： the control group, the high lipid group, the high lipid ＋ Vitamin D overload group, and the endothelium injury group. Then the content of TC, TG, HDL-C, and LDL-C and the activity of TAFI were determined by a ehromogenie assay. Results： Atheroselerosis was successfully established. Hyperlipoidemia stage, fiber hyperplasy arteriosclerosis stage and maturatly plaque stage were also success- fully duplicated. TC, TG, LDL-C and the activity of TAFI in the plasma of the three model groups gradually increased compared with the control group （ P 〈0.01）. However, HDL-C in the plasma of the three model groups gradually decreased compared with the control group（ P 〈0.01 or P 〈0.05）. The activity of TAFI in the plasma positively related to TG and TC. Conclusion： The activity of TAFI in the plasma is closely correlated with atheroselerosis severity.

关 键 词：动脉硬化 凝血酶活化的纤维蛋白溶解抑制剂 内皮 损伤 大鼠 Wistar 

分 类 号：R541.4[医药卫生—心血管疾病] R-332[医药卫生—内科学]