灰木相思蛋白质组双向电泳条件的优化  

Establishment of a Two-Dimensional Electrophoresis Protocol for Proteome of Acacia implexa

作　　者：何文锦[1] 郭晋隆[2] 陈由强[1] 林思祖[3] 陈如凯[1] 

机构地区：[1]福建师范大学生命科学学院,福州350007 [2]农业部甘蔗生理生态与遗传改良重点开放实验室,福州350002 [3]福建农林大学林学院,福州350002

出　　处：《西北植物学报》2007年第8期1577-1582,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：福建省自然科学基金资助项目(B0510009);福建省林业厅种苗科技攻关项目(闽林综[2003]17号)

摘　　要：针对灰木相思(Acacia implexa Benth.)蛋白质提取过程中含有大量色素和酚等干扰物质的现象,进行了实验条件的优化,结果显示:选择含较少次生物质量的组培苗为实验材料更易得到清晰的双向电泳图谱,在裂解液中添加硫脲更有利于提高蛋白溶解度,第一向等电聚焦固相pH梯度胶条(24 cm)的最适蛋白上样量为1000μg,表明采用优化后的双向电泳体系提高了灰木相思总蛋白双向电泳的分辨率和重复性,建立起一套适用于灰木相思总蛋白质组分析的双向电泳(2-DE)方法.A 2-DE（two-dimensional electrophoresis）protocol suited for the separation of proteins from Acacia implexaBenth, was established. Experiments shown that distinct 2-DE maps can be got when A. implexa plantlets in vitro were selected as experiment material. The lysis buffer adding triourea was helpful to improve the protein solubility. The optimum loading quantity of sample protein was 1 000μg in isoelectric focusing. The optimized protocol of A. implexa markedly minimized the interference from wood pigments and other non-protein substances.

关 键 词：灰木相思 蛋白质组学 双向电泳 

分 类 号：Q946-33[生物学—植物学]