不同处理对小麦×玉米产生小麦单倍体胚频率的影响  

Effects of Different Treatments on Frequency of Wheat Haploid Embryo Production through Wheat×Maize

作　　者：闫林[1] 王成社[1] 王辉[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100

出　　处：《西北植物学报》2007年第8期1593-1599,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家跨越计划项目(2006跨08)

摘　　要：在田间条件下,选用3个小麦材料与不同玉米品种进行杂交,用含不同体积分数二甲基亚砜(DMSO)的100 mg/L 2,4-D溶液和3%DMSO与2,4-D配比处理两种方法诱导小麦×玉米产生单倍体.结果表明,在不同DMSO处理中,2%体积分数的DMSO最佳处理平均得胚率最高为15.8%,3%处理最低(7.1%),二者差异显著;在激素配比试验中,以授粉后48 h采用小花滴注100 mg/L 2,4-D溶液、授粉后72 h再次采用小花滴注并穗下茎节注射3%DMSO的100 mg/L 2,4-D溶液的处理得胚率最高(14.1%),但与3%DMSO的2,4-D 1次处理(13.4%)2、,4-D 1次处理(12.7%)、2,4-D 2次处理(10.6%)和3%DMSO的2,4-D 2次处理(12.6%)之间无显著差异,而这5种处理的得胚率均与对照和未授粉只进行3%DMSO的2,4-D 1次处理存在极显著差异.In the field, three wheat varieties were crossed with different maize varieties. Two treatment methods were applied：Different volume concentrations of DMSO combination with 100 mg/L 2,4-D（treatment 1） and hormone combinations （treatment 2）. The results showed that various concentrations of DM- SO and hormone treatments exhibited different effects on the frequency of haploid embryo formation. In treatment 1,the optimal volume concentration of DMSO in 2,4-D is 2% （15.8%）, while the frequency of embryo formation with 3% DMSO is the lowest （7.1%）. The difference was significant. The best treatment for hormone combinations was that firstly dropping to the spikelets at 48 h after pollination with 100 mg/L 2,4-D and secondly dropping to the spikelets and simultaneously injecting into the stem at 72 h after pollination with 3 % DMSO in 2,4-D. The frequency of embryo formation was 14.1%. There were not significant differences among 2,4-D and 3. 0% DMSO combined treatment （14. 1%）, 3. 0% DMSO alone treatment （13.4%） ,2,4-D alone treatment （12.1%） ,2,4-D repeat treatments （10. 6%） and 3.0% DMSO repeat treatment （12.6 %）. However, there were extremely significant differences in the frequencies of embryo formation between these five treatments and control treatment or unpollinated 3.0% DMSO alone treatment.

关 键 词：小麦 玉米 单倍体 2 4-D 二甲基亚砜 得胚率 

分 类 号：Q321.3[生物学—遗传学]