不同时程电针对大鼠大脑中动脉阻塞再灌注区脑组织兴奋性氨基酸含量的影响  

Effect of Electroacupuncture on the Contents of Excitatory Amino Acids in Cerebral Tissue at Different Time Courses in Rats with Cerebral Ischemia and Reperfusion Injury

作　　者：张天生[1] 杨露[2] 胡荣[2] 乔秀兰[2] 杨馨[2] 刘旭光[2] 

机构地区：[1]山西省针灸研究所,太原030006 [2]成都中医药大学,成都610075

出　　处：《针刺研究》2007年第4期234-236,共3页Acupuncture Research

基　　金：四川省科技厅项目(05jy029-074-2)

摘　　要：目的:通过检测大鼠大脑中动脉阻塞(MCAO)再灌注区脑组织兴奋性氨基酸含量,比较不同时程电针对脑缺血再灌注损伤保护作用的差异,为缺血性中风选择最佳电针时间提供理论依据。方法:选用SD大鼠40只,随机分为5组,即假手术对照组、MCAO模型组、电针10 min组、电针30 min组、电针60 min组。制作可逆性脑缺血再灌注损伤大鼠模型,再灌注前不同时程电针"百会""水沟",刺激强度3.5 mA,频率2 Hz,连续波。高效液相法检测大鼠MCAO再灌注区脑组织谷氨酸(Glu)、天门冬氨酸(Asp)含量。结果:与模型组比较,电针各组梗死区脑组织Glu含量都明显降低(P<0.05);电针60 min时程能有效降低Asp含量(P<0.05)。结论:达到一定的电针时间才能更有效地保护脑缺血再灌注后的损伤。Objective： To observe changes of contents of excitatory amino acids in focal cerebral ischemic tissue after electroacupuncture （EA） treatment at different time courses in cerebral ischemla and reperfusion （CI/R） rats so as to provide experimental evidence for selecting the best duration of EA stimulation in the treatment of apoplexy. Methods： Forty SD rats were randomized into control, model, EA-10 min, EA-30 min and EA-60 min groups. CI/R model was established by middle cerebral artery occlusion （MCAO） and reperfusion. EA （2 Hz, 3.5 mA and continuous waves） was applied to ＂Baihui＂ （GV 20） and ＂Shuigou＂ （GV 26） for 10 min, 30 min and 60 min respectively. Cerebral glutamate （Glu） and aspartic acid （Asp） concentrations were detected by using an amino acid automatic analyzer after homogenate and centrifugalization of the right brain tissue. Results： Compared with control group, Asp and Glu contents of model group and EA-10 min group increased significantly （P〈0. 01 ） ; while in comparison with model group, Asp level in EA-60 min group, and Glu level in EA-10 min, EA-30 min and EA-60 min groups decreased considerably（P〈0.05）. No significant differences were found between control and EA-30 min groups, and between control and EA-60 min groups in Asp and Glu levels （ P〉0. 05）. Conclusion ： EA of GV20 and GV26 for 60 min can markedly reduce Glu and Asp levels in the topical cerebral infarct area in CI/R rats, which may contribute to its effect in relieving cerebral ischemic injury. Suitable duration of EA stimulation is definitely necessary for inducing a better therapeutic effect.

关 键 词：电针 大脑缺血再灌注损伤 GLU ASP 

分 类 号：Q493.2[生物学—生理学]