乙醇对大鼠生精细胞Bcl-2蛋白的影响与葛根素的干预  

Interventional effect of puerarin on Bcl-2 protein of ethanol-induced spermatogenic cells in rats

作　　者：邓仪昊[1] 陈道邦[1] 徐立[1] 史凯[1] 

机构地区：[1]四川大学华西医学中心人体解剖学教研室,四川省成都市610041

出　　处：《中国组织工程研究与临床康复》2007年第34期6801-6804,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察葛根素对乙醇所致睾丸损伤的保护作用,探讨其可能机制。方法:实验于2006-04-03/2007-02-20在四川大学人体解剖学教研室实验室进行。取SD成年雄性大鼠24只,随机分为3组:①乙醇组:给予白酒灌胃(乙醇体积分数为0.56),10mL/(kg·d)。②葛根素组:给予与乙醇组等量白酒灌胃后,立即腹腔注射葛根素62.5mg/(kg·d)。③对照组:给予10mL/(kg·d)生理盐水灌胃。给药连续3个生精周期共39d,于第40天麻醉后处死取睾丸,测定睾丸组织中超氧化物歧化酶活性及丙二醛含量;免疫组织化学法检测各组Bcl-2,Bax蛋白在生精细胞的表达情况;TUNEL法检测生精细胞的凋亡。结果:24只均进入结果分析。①乙醇组睾丸匀浆超氧化物歧化酶活性低于对照组(P<0.01),丙二醛含量高于对照组(P<0.01),葛根素组与对照组相比差异不显著(P>0.05)。②乙醇组平均每个生精小管断面中Bcl-2蛋白阳性细胞数和A值低于对照组(P<0.01),葛根素组与对照组比较差异不显著(P>0.05)。③乙醇组平均每个生精小管断面中Bax蛋白的阳性细胞数和A值高于对照组(P<0.01),但葛根素组与对照组比较差异不显著(P>0.05)。④乙醇组每个生精小管横切面中的凋亡细胞数目高于对照组(P<0.01),葛根素组与对照组比较差异不显著(P>0.05)。结论:乙醇通过氧化损伤作用使生精细胞发生凋亡,葛根素有对抗乙醇所致睾丸损伤的作用。AIM： To investigate the effect of puerarin on protecting against ethanol-induced injury in rat testis. METHODS： The experiment was carried out in the laboratory of Human Anatomy Department in Sichuan University from April 3^th 2006 to February 20^th 2007. Twenty-four SD adult male rats were divided into three groups： ①Group A： The rats were lavaged with 10 mL/kg liquor of 56% alcohol dairy; ②Group B： The rat was treated with 10 mL/kg liquor of 56% alcohol and 62.5 mg/kg puerarin by intraperitoneal injection;③Group C： The rat was treated with equal volume of NaCI. All the rats were treated for 39 days continuously in three spermatogenic cycles. At the 40^th day, testis tissues were obtained after anesthesia to measure the contents of superoxide dismutase （SOD） and malondialdehyde （MDA）; expressions of Bcl-2 and Bax in spermatogenic cells were checked by immunohistochemistry; Apoptosis of spermatogenic cells was determined by TUNEL. RESULTS： All of the 24 rats were involved in the result analysis.①SOD activity in group A was lower than that in group C （P 〈 0.01）, but MDA was adverse （P 〈 0.01）. There was no significant statistical difference between group B and group C （P 〉 0.05）. ②The expression of Bcl-2 and A value in seminiferous tubule section were lower in group A than in group C （P 〈 0.01）, and there was no significant statistical difference between group B and group C （P 〈 0.05）.③The expression of Bax and A value in seminiferous tubule section were higher in group A than in group C （P 〈 0.01）, and there was no significant statistical difference between group B and group C （P 〈 0.05）. ④Apoptosis of spermatogenic cells in group A was higher than that of group C （P 〈 0.01）, but there was no significant statistical difference between group B and group C （P 〈 0.05）. CONCLUSION： Spermatogenic cells generate apoptosis by oxidative damage of alcohol. Puerarin can inhibit oxidative damage of didymus by alcohol.

关 键 词：乙醇 睾丸 葛根素 

分 类 号：R697[医药卫生—泌尿科学]