广西环江县毛南族九个短串联重复序列基因座的遗传学分析  

A Study of Polymorphisms of 9 STR Loci in the Maonan Ethnic Group of Huanjian,Guangxi Province

作　　者：周丽宁[1] 李松峰[1] 徐林[1] 龚继春[1] 邓琼英[1] 

机构地区：[1]广西医科大学解剖教研室人类学研究室,南宁530021

出　　处：《人类学学报》2007年第3期264-269,共6页Acta Anthropologica Sinica

基　　金：国家自然科学基金资助项目(30260044)

摘　　要：为了了解广西环江毛南族人群无关个体的九个短串联重复序列:vWA,D18S51,D5S818,FGA,D8S1179,D21S11,D7S820,D3S1358,D13S317基因座的遗传多态性分布情况;本文用枸橼酸钠抗凝法采集广西环江县毛南族200份无亲缘关系的健康个体的血样,Chelex-100方法提取DNA,应用AmpFlSTRIdentifilerTM荧光标记复合扩增技术对血样DNA的九个STR基因座进行扩增,用ABI 3100型遗传分析仪对扩增产物进行检测。结果显示九个STR位点的基因型分布均符合Hardy-Weinberg平衡定律,累积非父排除率达0.999996,累积个体识别能力达0.99999999996,多态信息总量为0.9999985。结论:广西环江县毛南族人群有自身的STR等位基因分布特征,所获数据可为法医学个体识别、亲子鉴定及群体的遗传学研究提供依据。To investigate the distribution of nine short tandem repeat （STR） loci in the Maonan ethnic group from Guangxi Huanjiang. We collected the sodium citrated-blood specimens from 200 healthy unrelated Maonan individuals in Guangxi Huanjiang, and used the Chelex-lO0 method to extracte DNA by using AmpFeSTR IdentifilerTM PCR amplification kit and 3100 genetic analyzer. In the study, a total of 37 alleles and 170 genotypes of the nine short-tandem repeat loci were found in 200 specimens. The allele frequency and genotype frequency were 0. 0025-0.4600 and 0. 0050-0. 2300, respectively. The expected distribution of this genotype accords with Hardy-Weinberg equilibrium（ P 〉 0.05）. The total discrimination power （ TDP ）, total polymorphism information （ TPI ）, cumulative paternity exclusion power（ECP） and average heterozygosity were 0. 99999999996, 0.9999985, 0.999996 and 0. 8028, respectively. These results demonstrate that these nine STR loci are of high polymorphic value and hereditary stability, and are in accord with Mendel＇s law. The data obtained are valuable for rearch in population genetics research, forensic application, and individual identifications.

关 键 词：STR 基因频率 遗传距离 毛南族 

分 类 号：Q987[生物学—遗传学]