NaCl胁迫及钙调节对青钱柳根部组织离子分布的影响  

Effect of NaCl stress and Ca^(2+) regulation on ion distribution in root tissues of Cyclocarya paliurus seedling

作　　者：姚瑞玲[1] 方升佐[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《植物资源与环境学报》2007年第2期22-26,共5页Journal of Plant Resources and Environment

基　　金：江苏省高新技术研究计划项目(BG2006314);国家自然科学基金资助项目(30371156)

摘　　要：运用X-射线微区分析法研究了NaCl胁迫及钙调节下,青钱柳〔Cyclocarya paliurus(Batal.)Iljinskaja〕幼苗根部组织中离子的浓度和分布情况。结果表明,3.0和5.0 g.L-1NaCl胁迫下,青钱柳幼苗根部各层组织中Na+和Cl-的相对含量增加,K+、Ca2+和Mg2+的相对含量降低;5.0 g.L-1NaCl胁迫下加入3.0 g.L-1Ca(NO3)2后,Na+的相对含量降低,K+的相对含量增加,Cl-的相对含量变化较小。随NaCl浓度的升高,中柱和皮层中Cl-的相对含量增高;Na+大部分沉积在皮层及表皮上;K+由皮层进入中柱的比率增大;Ca2+由表皮进入皮层的比率减少,由皮层进入中柱的比率增大。5.0 g.L-1NaCl胁迫下加入高浓度Ca(NO3)2后,Cl-的分布比率变化不明显;Na+由表皮进入皮层的比率增加,从皮层进入中柱的比率下降;K+和Ca2+的分布比率变化不明显。高浓度NaCl胁迫破坏了青钱柳幼苗根部的离子平衡,加入外源Ca(NO3)2(1.0~3.0 g.L-1)后,缓解作用不明显。The ion concentration and distribution in root tissues of Cyclocarya paliurus （ Batal. ） Iljinskaja seedlings under NaCl stress and Ca^2＋ regulation were studied by X-ray microanalysis technique. The results showed that under 3.0 and 5.0 g·L^-1 NaCl stress, relative contents of Na^＋ and Cl^- in different tissues of root increased, and that of K^＋ , Ca^2＋ and Mg^2＋ decreased. After treated by 5.0 g·L^-1 NaCl stress and 3.0 g·L^-1 Ca（ NO3 ） 2, relative content of Na^＋ reduced and that of K^＋ increased, and the variation of Cl^- relative content was very slightly. With increasing of NaCl concentration, Cl^- relative contents in stele and cortex increased, most of Na^＋ aggregated to cortex and epidermis. Ratio of K^＋ transporting from cortex into stele increased, while ratio of Ca^2＋ from epidermis into cortex decreased and from cortex into stele increased. Under 5.0 g·L^-1 NaCl stress with high concentration of Ca （ NO3 ）2, the variation of Cl^- distribution ratio was not evident, Na^＋ transporting from epidermis to cortex increased whereas reduced from cortex into stele, and variation of K^＋ and Ca^2＋ distribution ratio was not apparent. It is concluded that high concentration of NaCl stress disruptes ion balance in root of C. paliurus seedlings and regulation effect of exogenous calcium （ 1.0 - 3.0 g·L^-1 ） is not significant.

关 键 词：NACL胁迫 钙调节 青钱柳 离子分布 X-射线微区分析 

分 类 号：Q945.78[生物学—植物学] S792.12[农业科学—林木遗传育种]