授粉后施加秋水仙碱处理诱导青杨派异源三倍体  被引量:12

Induction of allo-triploid plants in Populus(Section Tacamahaca) with colchicine after pollination

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作  者:王君[1] 康向阳[1] 李代丽[1] 景艳春[1] 杨伟光 

机构地区:[1]北京林业大学林木花卉遗传育种教育部重点实验室

出  处:《北京林业大学学报》2007年第5期18-21,共4页Journal of Beijing Forestry University

基  金:国家自然科学基金项目(30671708)

摘  要:从中国北方地区拓展杨树品种资源考虑,以哲引3号杨为母本,小青杨和北京杨为父本,对授粉后24~96 h的雌花序施加0.3%~0.5%的秋水仙碱溶液处理,获得了26株青杨杂种三倍体。其中尤以授粉后54~66 h时施加秋水仙碱处理的三倍体诱导效果最好,三倍体得率最高,达66.7%。与二倍体后代群体相比,青杨杂种三倍体具有一定的生长优势,其中哲引3号杨×北京杨三倍体2年生实生苗的地径和苗高平均值分别超出二倍体20%和5%;最优单株的地径和苗高分别达4.42 cm和2.77 m,超出二倍体平均值144%和43%。青杨派树种的三倍体诱导实践进一步证明,雌花序授粉后施加秋水仙碱处理是一种高效的三倍体诱导方法。 In order to enrich the resources of Populus varieties in north China,female catkins were treated with 0.3%-0.5% colchicine solution during 24-96 hrs after pollination in cross combinations including(P.pseudo-simonii × P.nigra var.lica) ×(P.× beijingensis) and(P.pseudo-simonii × P.nigra var. lica) × P.pseudo-simonii,and 26 triploid plants were obtained.The results showed that 54-66 hrs after pollination was the optimal period to induce triploids in these cross combinations,and the highest ratio of obtaining triploids was 66.7%.Compared with the diploid progeny,the growth of these allo-triploids showed certain superiority.Especially in(P.pseudo-simonii × P.nigra var.lica) ×(P.× beijingensis),on average,the basal diameter and stem height of two-year-old triploid seedlings exceeded the ones of diploids by 20% and 5% respectively;the two indices of the best triploid in this cross combination were 4.42 cm and 2.77 m,which were 144% and 43% more than those of the average growth of diploids respectively.Induction of allo-triploids in Section Tacamahaca further proves that treating pollinated female catkins with colchicine is an efficient method to obtain triploid plants.

关 键 词:哲引3号杨 青杨派 授粉 秋水仙碱 三倍体 

分 类 号:S792.11[农业科学—林木遗传育种] S718.46[农业科学—林学]

 

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