珍稀植物香果树植株再生体系的建立  

Establishment of in vitro plant regeneration system of rare endangered plant Emmenopterys henryi Oliv.

作　　者：熊丹[1] 陈发菊[1] 梁宏伟[1] 王玉兵[1] 李凤兰[2] 

机构地区：[1]三峡大学生物技术研究中心 [2]北京林业大学生物科学与技术学院

出　　处：《北京林业大学学报》2007年第5期44-49,共6页Journal of Beijing Forestry University

基　　金：湖北省生物资源保护与利用重点实验室开放基金(2007010);国家林业局自然保护区研究中心项目(460--8101);宜昌市科技重点攻关项目(A06209)

摘　　要：通过不定芽直接再生和经愈伤组织诱导器官发生两种途径,建立了香果树快速、高频率发生的植株再生体系,探讨了不同外植体(幼叶切块、茎段、叶柄和根)、叶片放置方式、植物生长调节剂和培养条件对不定芽直接再生和愈伤组织诱导器官发生的影响.香果树愈伤组织诱导器官发生结果表明:不同外植体在MS附加1.0 mg/L 6-BA和1.0 mg/L 2,4-D的培养基上愈伤组织诱导率均为100%,其中叶片外植体叶面向下放置效果最好;愈伤组织在MS添加0.5 mg/L 6-BA和0.5 mg/L NAA的培养基上不定芽的分化率为94.35%.香果树不定芽直接再生结果显示:叶片外植体在MS添加6-BA或ZT的培养基上,不定芽直接再生率均高达100%,其中在MS附加2.0 mg/L 6-BA培养基上,产生的不定芽数目多,生长旺盛;附加1.0 mg/L ZT培养基上,不定芽数目多达56.67个/cm2,但生长较弱.黑暗预培养有利于香果树叶片外植体不定芽的再生.两种途径得到的不定芽转到1/2 MS培养基上均可生根,生根率达100%,附加1.0 mg/L的IBA和0.5%的活性炭有利于再生植株根的生长.　A rapid and effective system of plant regeneration via direct shoot regeneration and organgenesis in the endangered tree of Emmenopterys henryi Oliv.was optimized by studying the influence of explant types（root,stem,petiole and leaf）,disposition of leaf,plant growth regulators（PGR） and culture conditions.The optimum callus induction and organgenesis were achieved when the upper surface of leaf explants was placed downward in the medium： 100% callusing was achieved in MS medium supplemented with 1.0 mg/L 6-BA and 1.0 mg/L 2,4-D,and 94.35% differentiation along with a multiplication rate of 1.95 per explant with a combination of 0.5 mg/L 6-BA and 0.5 mg/L NAA. In the direct shoot regeneration system,the adventitious buds were obtained using leaves as explants in 40 days following the incubation on MS medium supplemented with 2.0 mg/L 6-BA or 1.0 mg/L ZT,the frequency of adventitious buds was as high as 100%.The average number of buds（height≥5 mm） per explant was 15 and that of height〈5 mm was 56.67 per square centimeter,respectively.At the same time,dark periods could facilitate shoot regenerate.The plants from direct shoot regeneration and organgenesis developed roots on 1/2 MS medium at an efficiency of 100%.Adding 0.5% activated carbon（AC）and 1.0 mg/L IBA could promote the development of rooting.The plantlets were transferred successfully to the greenhouse with a high survival rate of 95%.

关 键 词：香果树 愈伤组织 植株再生 不定芽 

分 类 号：S718.46[农业科学—林学]