自发性高血压大鼠第三脑室触液神经元内一氧化氮合酶与加压素的共表达  

Coexistence of nitric oxide synthase with vasopressin in cerebrospinal fluid-contacting neurons of the third ventricle in spontaneously hypertensive rat

作　　者：张露青[1] 肖明[1] 吴凌霞[1] 王鹤鸣[1] 丁炯[1] 

机构地区：[1]南京医科大学人体解剖学系,南京210029

出　　处：《解剖学杂志》2007年第4期452-455,共4页Chinese Journal of Anatomy

基　　金：江苏省自然科学基金(BK2006232)

摘　　要：目的:观察自发性高血压大鼠(SHR)与SD大鼠第三脑室触液神经元(CSFCN)内一氧化氮合酶(NOS)与加压素(VP)的分布和共存。方法:应用还原型尼克酰胺嘌呤二核苷酸磷酸脱氢酶(NADPH-d)组织化学方法,结合ABC免疫组织化学技术。结果:在SHR视前区至室间核后大细胞亚核平面的第三脑室室壁均有NOS阳性CSFCN的分布;在SHR第三脑室室壁的CSFCN内NOS与VP具有共存性,且SHR组第三脑室的CSFCN内NOS与VP共存率较SD组高。结论:一氧化氮(NO)与VP在下丘脑的血压神经内分泌活动调节中起着重要的介导作用,也可能对高血压的发生发展有影响。Objective： To investigate the coexistence of nitric oxide synthase （NOS） with vasopressin （VP） in the cerebrospinal fluid-contacting neurons （CSFCN） of the third ventricle （3V） in spontaneously hypertensive rats （SHR）. Methods： The morphology and distribution of NOS positive neurons and VP immunoreactive neurons were observed in CSFCN of both SHR and normal rats, and the coexistence of NOS with VP was also observed by using NADPH-diaphorase histochemical technique combined with immunocytochemical methods in the same section. Results： The coexistence of NOS with VP was found in CSFCN of SHR. The percentage of double-labeled neurons to total labeled neurons in SHR was higher than that in SD group. Conclusion： These results suggest that NO and VP may play an important role in modulating blood pressure of the hypothalamic neuroendocrine regulations, and may he related to the occurrence and development of hypertension.

关 键 词：自发性高血压 一氧化氮合酶 加压素 第三脑室 触液神经元 

分 类 号：R544.1[医药卫生—心血管疾病]