离子对色谱法测定注射用氨苄西林钠氯唑西林钠的含量  

Determinatin of Ampicillin Sodium and Cloxacillin Sodium in Injection by Ion-pairs HPLC Method

作　　者：陈汝红[1] 董哲卿[1] 鹿颐[1] 

机构地区：[1]河北省药品检验所,石家庄050011

出　　处：《中国现代应用药学》2007年第4期308-310,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 建立离子对色谱法测定注射用氨苄西林钠氯唑西林钠含量的方法.方法 采用C18柱,缓冲盐溶液（内含0.01 mol·L^-1十六烷基三甲基溴化铵和0.01 mol·L^-1磷酸二氢钾）（用氢氧化钾试液调节pH值为6.5）-乙腈（50∶50）为流动相,流速1.0 mL·min^-1,检测波长230 nm.结果 氨苄西林和氯唑西林的线性范围分别为0.06～1.67 mg·mL^-1和0.06～1.54 mg·mL^-1,r均为0.999 9;平均回收率分别为99.9%和99.8%.结论 本法简便、快速、准确,可用于注射用氨苄西林钠氯唑西林钠的含量测定.OBJECTIVE To establish an ion-pairs HPLC method for the assay of ampicillin sodium and cloxacillin sodium in injection. METHODS The column： C 18;The mobile phase： a mixture of buffer solution（ containing of 0.01 mol · L^-1 cetyl trimethyl ammonium bromide and 0.01 mol · L^-1 potassium dihydrogen orthophosphate,adjust pH to 6.5 with potassium hydroxide solution）and acetonitrile （50：50） ;The flow rate：1.0 mL · min^-1 ;The detection wavelength： 230 nm. RESULTS The linear range of ampicillin and cloxacillin was 0.06 - 1.67 mg· mL^-1 and 0.06 - 1.54 mg · mL^- 1 respectively, r = 0.999 9. The average recovery was 99.9% and 99.8% respectively. CONCLUSION The proposed HPLC method was convenient, rapid and accurate, and could be used for assay of ampicillin sodium and cloxacillin sodium in injection.

关 键 词：氨苄西林钠氯唑西林钠 含量 离子对色谱法 

分 类 号：R917.101[医药卫生—药物分析学] R917.791[医药卫生—药学]