Echistatin对人增生性瘢痕成纤维细胞的作用研究  

Research of echistatin effect on human hypertrophic scar-derived fibroblasts

作　　者：范鹏举[1] 李珍[2] 黄晓元[1] 

机构地区：[1]中南大学湘雅医院烧伤整形科,湖南长沙410008 [2]中南大学湘雅二院ICU,湖南长沙410011

出　　处：《中国医学工程》2007年第6期469-472,共4页China Medical Engineering

摘　　要：目的研究Echistatin对人增生性瘢痕成纤维细胞增殖、凋亡及Ⅰ、Ⅲ型前胶原mRNA表达的影响。方法体外培养人增生性瘢痕成纤维细胞,设Echistatin处理组和对照组,MTT法、流式技术及RT-PCR法检测人增生性瘢痕成纤维细胞增殖、凋亡及Ⅰ、Ⅲ型前胶原mRNA的表达。结果Echistatin可明显抑制人增生性瘢痕成纤维细胞增殖,促进凋亡,降低Ⅰ、Ⅲ型前胶原mRNA的表达。结论Echistatin可作为防治增生性瘢痕的筛选药物。[Objective] To investigate the effect of echistatin on proliferation, apoptosis, and type Ⅰ and type Ⅲprocollagen mRNA expression in human hypertrophic scar-derived fibroblasts. [Methods] After treated by eehistatin, proliferation, apoptosis, type Ⅰ/type Ⅲ procollagen mRNA expression in cultured fibroblasts from human hypertrophic sear were determined by MTT method, Flow eytometry , and RT-PCR respectively. [Results] Eehistatin treatment resulted in significant inhibition of proliferation, type Ⅰ/type Ⅲ proeollagen mRNA expression, and marked apoptosis in cultured hypertrophic scar-derived fibroblasts. [Conclusion] Eehistatin may be chosen as a drug to prevent and cure hypertrophic scar.

关 键 词：ECHISTATIN 成纤维细胞 瘢痕 

分 类 号：R392.28[医药卫生—免疫学]