雷公藤多甙对类风湿关节炎患者滑膜细胞凋亡的影响  

Effects of tripterygium glycosides on synoviocyte's apoptosis of rheumatoid arthritis

作　　者：周光伟 沈权[2] 金若珏 王晓东 曾云祥 叶澄宇[2] 

机构地区：[1]浙江省温州市第八人民医院,325000 [2]温州医学院附属第一医院,325000

出　　处：《浙江临床医学》2007年第8期1034-1035,共2页Zhejiang Clinical Medical Journal

基　　金：温州市鹿城区科学技术局资助项目(S040210)

摘　　要：目的观察雷公藤多甙(Tripterygium Glycosides,TG)对类风湿关节炎(rheumatoidarthritis,RA)患者滑膜细胞凋亡的影响,以揭示TG治疗RA可能的作用机制。方法体外培养RA患者滑膜细胞,加入不同浓度的TG,24h光镜观察其凋亡的形态学特征,并用流式细胞仪及TUNEL法检测滑膜细胞的凋亡。结果TG组凋亡细胞百分率(55±11.2)%,显著高于骨折(NC)组(P<0.01)。结论TG可诱导滑膜细胞凋亡,这可能是其治疗RA的作用机制之一。Objective To observe the effects of tripteryglum glycosides（TG） on synoviocyte＇ s apoptosis of rheumatoid arthritis（RA） , in order to explore the possible mechanism of TG in treating RA . Methods Synoviocyte from RA patients were cultured in vivo , different concentrations of TG were added , the morphological charactors of synoviocyte＇s apaptosis was observed under light microscope 24 hours later, flow cytometry and TUNEL were used to detect the apoptosis of synoviocyte , Results The apoptosic cells was 55 % ~ 11.2% in TG group , which was significantly higher than that in bone fracture group（ P 〈 0.01 ）. Conclusios TG could induce the apaptosis of synoviocyte which may be one of its mechanism for treating RA.

关 键 词：类风湿关节炎 滑膜 雷公藤多甙 凋亡 

分 类 号：R259[医药卫生—中西医结合]