小白菜小孢子胚状体诱导成苗及利用  

The Establishment and Application of Isolated Microspore Culture Technology System in Brassica campestris L. ssp. chinensis var. communis Tsen et Lee

作　　者：王超楠[1] 冯辉[2] 姜凤英[2] 刘如娥[2] 周英[2] 

机构地区：[1]沈阳农业大学农学院,110161 [2]沈阳农业大学园艺学院,110161

出　　处：《中国蔬菜》2007年第8期18-21,共4页China Vegetables

基　　金：国家自然科学基金(30671414);国家"863"项目(20060110Z1072)

摘　　要：以10个小白菜F1为试材进行游离小孢子培养,研究小孢子胚发生及其成苗的主要影响因素,并对获得的双单倍体以及用其配制的杂交组合的园艺学性状进行鉴定。结果表明,品种间小孢子出胚率差异较大;NLN-13培养基中大量元素减半有利于小孢子胚发生,胚诱导率达100%;培养基中添加适量的6-BA和NAA可以提高小孢子出胚率;小孢子胚成苗培养基的适宜琼脂浓度为1.0%,小孢子胚成活率可达85.88%;MS+3%蔗糖+0.8%琼脂是小孢子再生植株继代和壮苗的适宜培养基,MS+3%蔗糖+0.8%琼脂+NAA0.1mg.L-1是小孢子再生植株生根的适宜培养基;双单倍体及其与自交系配成的杂交组合整齐一致。Isolated microspore culture was carried out with ten Fl hybrids of pakchoi （ Brassica campestris L. ssp. chinensis var. communis Tsen et Lee. ）. The frequency of embryogenesis, embryos germinated seedling, subculture and rootage of regenerated plantlets, uniform identification of horticultural characters from the DH line progenies and hybrid made with DH lines were studied. The results showed that genotypes had remarkable effect on embryoid induction. Using semicontent of macro-elements in NLN - 13 medium increased the embryoid induction and the frequency of embryogenesis reached 100 %. The suitable concentration of 6 - BA and NAA remarkably enhanced the frequency of embryogenesis. MS medium with 1.0 % agar content was optimal for plantlet regeneration, survival rate of embryo was 85.88 %. MS ＋ 3 % sucrose ＋ 0. 8 % agar was the optimal medium for subculture and renewal of pakchoi. MS ＋ 3 % sucrose ＋ 0. 8 % agar ＋ NAA 0. 1 mg · L^ -1 was the suitable medium for rooting. Analysis of horticultural characters indicated that the DH lines and hybrid made with them were uniform.

关 键 词：小白菜 小孢子培养 胚状体 双单倍体 

分 类 号：S634.3[农业科学—蔬菜学]