内皮祖细胞移植在四氯化碳诱导的大鼠肝纤维化模型中的作用  

The effects of endothelial progenitor cell transplantation in carbon tetrachloride induced hepatic fibrosis rats

作　　者：刘峰[1] 费然[1] 饶慧瑛[1] 丛旭[1] 哈明昊[1] 魏来[1] 

机构地区：[1]北京大学人民医院、北京大学肝病研究所,100044

出　　处：《中华肝脏病杂志》2007年第8期589-592,共4页Chinese Journal of Hepatology

基　　金：国家“863”计划（2001AA216031）;高等学校博士学科点专项科研基金（20040001133）

摘　　要：目的研究内皮祖细胞（EPCs）移植对CCl4诱导的大鼠肝纤维化的影响。方法选用雌性健康SD大鼠,共24只。采用25％的CCl4/橄榄油灌胃8周制成SD大鼠肝纤维化模型,随机处死8只为CCl4处理8周组,再随机取16只分成EPCs回输组和等渗盐水对照组,每组各8只。在EPCs回输组,EPCs经门静脉回输入大鼠体内；等渗盐水对照组,门静脉回输入等体积的等渗盐水,回输4周后处死所有大鼠,取肝组织石蜡切片,进行苏木精-伊红常规染色和Masson三色染色法；免疫组织化学染色检测α-平滑肌肌动蛋白（α-SMA）和CⅢ,进行组织学和纤维化评估,同时取血清进行生化检测评估肝功能。结果与等渗盐水对照组相比,EPCs回输组中,肝组织学活动指数（HAI）,ALT、AST和TBil水平降低,Alb水平高,α-SMA和CⅢ表达少（P＜0．05）；与CCl4处理8周组相比,EPCs回输组中,HAI、ALT、AST和TBil水平降低,其他指标差异无统计学意义；等渗盐水对照组中HAI、ALT、AST和TBil水平升高,Alb水平降低（P＜0．05）。结论在肝纤维化发展过程中,门静脉回输EPCs,可以减轻肝脏炎症损伤,同时改善CCl4诱导的肝纤维化程度。Objective To study the effects of rat endothelial progenitor cell （EPC） transplantation on hepatic fibrosis in carbon tetrachloride （CCI4） induced hepatic fibrosis rats. Methods Hepatic fibrosis was developed in 24 healthy female SD rats by feeding them 25% CCLdolive oil for 8 weeks. Eight of them were sacrificed at the end of the 8 weeks. The rats were subdivided into a EPCs transplanting group （n = 8） and a saline control group （n = 8）. After the EPCs were isolated and cultured for 9 days, the cells were injected into the portal veins of the rats in the EPCs transplanting group. Four weeks later all of the rats were sacrificed. The blood biochemical parameters from the serum were examined. The degree of liver fibrosis was evaluated by reading Masson staining liver slides and by detecting the expression of α -SMA and collagen Ⅲ. Results Compared with the saline control group, hepatic activity index （HAI）, levels of ALT, AST and TBil in the serum were all lower in the EPCs transplanting group, but the level of Alb was higher and the expression of α-SMA and collagen Ⅲ were lower （P 〈 0.05）. Compared with the 8 week hepatic fibrosis group, the levels of ALT, AST and TBil in the serum of the EPCs transplanting group were all lower. In the saline control group, the serum levels of ALT, AST and TBil were higher, the level of Alb was lower, and the expressions of ct -SMA and Collagen Ⅲ were higher （P 〈 0.05）. Conclusion In hepatic fibrosis rats, transplantation of rat EPCs could minimize the hepatic fibrosis process and the injuries.

关 键 词：肝纤维化 内皮祖细胞 大鼠 

分 类 号：R686[医药卫生—骨科学]