心得力对心肌病大鼠心功能及心脏连接蛋白43表达的影响  

Effects of Xindly on heart function and myocardial connexin 43 protein expression in cardiomyopathy rats

作　　者：刘平[1] 程功[2] 强华[1] 韩克[1] 吴格如[1] 马爱群[1] 

机构地区：[1]西安交通大学第一附属医院心内科,陕西西安710061 [2]陕西省人民医院心内科,陕西西安710045

出　　处：《第四军医大学学报》2007年第16期1449-1451,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30600835)

摘　　要：目的:研究心得力(Xindly)对阿霉素(ADR)心肌病大鼠心功能及心脏连接蛋白43(Cx43)的影响,部分探讨其治疗心肌病的分子基础.方法:SD大鼠50只随机分为正常组,ADR心肌病心衰模型组(含安慰剂组,达爽组和心得力+达爽组).药物干预4wk用Hp Sonos2500型多功能超声检查仪无创检测各组大鼠心脏射血分数(EF),左心室舒张末内径(LVDD),左心室收缩末内径(LVDS);FITC标记抗Cx43的免疫组化法染色,激光共聚焦显微镜测量Cx43荧光强度,并作图像定量分析.结果:心得力+达爽组心功能较安慰剂组改善,左室收缩末内径较达爽组改善(P<0.05).心得力+达爽组Cx43的Area,PerArea,IOD均好于安慰剂组(P<0.05);心得力+达爽组Cx43的PerArea与西药组有统计学差异(P<0.05).结论:心得力+达爽治疗心肌病有疗效.AIM： To investigate the effects of Xindly on heart function and myocardial connexin 43 （ Cx43 ） protein expression in doxorubicin-induced cardiomyopathy rats , and to explore partly its molecular basis for treatment of cardiomyopathy. METHODS ： Fifty male SD rats were randomly divided into 4 groups： normal group, placebo group, imidapril group and Xindly ＋ imidapril group. After intervention for 4 weeks, ejection fraction （ EF ） , left ventricular diastolic dimension （LVDD） and left ventricular endsystolic dimension （LVDS）were measured by Hp Sonos 2500 ultrasound inspectoscope; FITC-labeled anti-Cx43 antibody was used in immunohistochemical technology with a quantitative imaging system. RESULTS： The heart function was significantly improved in xindly ＋ imidapril group than in placebo group. LVDS was better in xindly ＋ imidapril group than in imidapril group（ P 〈 0.05）. The Per Area, Area, integrated optical density（IOD） of myocardial Cx43 in xindly ＋ imidapril group were better than that in placebo group （ P 〈 0.05 ）. The Per Area in xindly ＋ imidapril group was better than those in imidapril group （ P 〈 0.05）. CONCLUSION ： Xindly ＋ imidapril administration is effective for the treatment of cardiomyopathy.

关 键 词：心肌疾病 心脏功能 连接蛋白43 心得力 

分 类 号：R542.2[医药卫生—心血管疾病]