周期性牵张肺泡Ⅱ型上皮细胞早期凋亡现象  

Early apoptosis induced by mechanical stretch in human alveolar type Ⅱ epithelial cells

作　　者：宁巧明[1] 王祥瑞[1] 

机构地区：[1]上海交通大学医学院仁济医院麻醉科

出　　处：《上海交通大学学报（医学版）》2007年第8期961-964,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市"百人计划"(03-77-20)~~

摘　　要：目的应用细胞力学的方法探讨周期性牵张肺泡Ⅱ型上皮细胞，并观察其早期凋亡现象。方法周期性牵张肺泡Ⅱ型上皮细胞株A549，首先观察细胞在不同机械力作用下的凋亡情况，分为对照组和机械牵张组（5％、15％、30％应力，0．5Hz，机械牵张4h）。然后给予细胞应力15％、0．5Hz、机械牵张0～8h，观察在不同时间点的细胞凋亡。最后给予细胞应力15％，周期性牵张4h，牵张频率分别为0、0．2、0．5和1Hz，观察不同周期频率的机械牵张时细胞凋亡情况。采用流式细胞仪检测细胞凋亡。结果周期性牵张肺泡上皮细胞A549、0．5Hz和机械牵张4h后，细胞早期凋亡随着牵张应力的增加而增加。当给予细胞15％应力和0．5Hz的机械牵张后，随着牵张时间的延长，早期凋亡细胞的比率增加。给予细胞15％应力，周期性牵张4h，随着牵张频率的增加早期凋亡的细胞也显著增加。结论机械应力刺激肺泡Ⅱ型上皮细胞可导致细胞凋亡，这在细胞力学水平为机械通气所致肺损伤提供了实验依据。To investigate the apoptosis response to mechanical stretch in human alveolar type Ⅱ epithelial cells A549. Methods First, A549 cells were given different stretch （0% , 5% , 15% , 30% ） at frequency of 0.5 Hz for 4 h. Then, A549 cells were stretched for different periods （0 h, 2 h, 4 h, 8 h） at stretch 15% ,0.5 Hz. Last, A549 cells were stretched for different frequency （0 Hz, 0.2 Hz, 0.5 Hz and 1 Hz） at stretch 15% for 4 h. The early apoptosis was detected by flow cytometry. Results A549 cells submitted to cyclic stretch caused the early apoptosis in a time - , stretch- and frequency-dependent manner. In details, at 0.5 Hz and 4 h stretch-dependence occurred, at 15% stretch and 0.5 Hz time-dependence occurred, and at 15% stretch and 4 h frequency-dependence occurred. Conclusion Mechanical stretch can induce the early apoptosis in human alveolar type Ⅱ epithelial cells A549, which may be one of the mechanisms of ventilator-induced lung injury.

关 键 词：肺泡Ⅱ型上皮细胞 机械牵张 凋亡 

分 类 号：R563.8[医药卫生—呼吸系统]