异丙酚对大鼠肺动脉平滑肌细胞钾通道电流的影响  

Effects of propofol on potassium currents in pulmonary artery smooth muscles of rats

作　　者：姜雨鸽[1] 徐龙河[1] 张宏[1] 

机构地区：[1]中国人民解放军总医院麻醉科,北京100853

出　　处：《中国临床药理学与治疗学》2007年第7期756-759,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(30300327)

摘　　要：目的:评价异丙酚对正常血压及肺动脉高压大鼠肺动脉平滑肌K+通道的影响。方法:用SD大鼠建立肺动脉高压大鼠模型。急性分离正常血压和肺动脉高压大鼠肺内动脉平滑肌细胞,用膜片钳全细胞记录技术记录细胞K+通道电流。通过浴槽内给药。结果:在50、100μmol/L浓度下,异丙酚可明显引起正常血压和肺动脉高压大鼠肺动脉平滑肌细胞外向K+通道电流I-V曲线上移。与对照组比较,异丙酚作用后5min内细胞外向K+通道电流强度分别增强为初始电流强度的(121±11)%、(113±5)%(P<0.01)和(126±5)%、(122±8)%(P<0.01)。结论:异丙酚可增强正常血压及肺动脉高压大鼠动脉平滑肌细胞的外向K+通道电流,这可能是其降低肺血管张力、舒张肺血管的机制之一。AIM： To investigate the effects of propofol on potassium currents in pulmonary artery smooth muscle cells of normotensive and hypertensive rats. METHODS： The effects of propofol on potassium currents in smooth muscle cells derived from nonnotensive and hypertensive rats pulmonary arteries were observed by patch clamp technique （whole cell recording） after application of the drug in the bath. RESULTS： The potassium current-voltage curves （I-V curves） of smooth muscle cells derived from normotensive and pulmonary hypertensive rats pulmonary arteries were up-ward shifted by propofol （50, 100μmol/L）. Compared with control group, within 5 minutes after application of the drug, the current amplitude could increase to （121 ± 11）%, （113±5）% （P〈 0.01） and （126±5）%, （122±8）% （P〈0.01） of initial current amplitude respectively. CONCLUSION： The out-ward potassium currents in smooth muscle ceils derived from normotensive and pulmonary hypertensive rats could be increased by propofol. This may be one of the mechanisms that propofol can decrease pulmonary vascular tension and dilate pulmonary vessel.

关 键 词：异丙酚 钾通道 平滑肌 血管 肺动脉 

分 类 号：R965.2[医药卫生—药理学]