马齿苋水提液保护衰老小鼠DNA端粒长度缩短的实验研究  

Effects of water extract of purslane herb on protecting telomere length of senile mouse

作　　者：黄浩[1] 余南才[1] 刘倩[1] 易艳东[1] 马威[1] 

机构地区：[1]武汉第一医院实验中心

出　　处：《中国临床药理学与治疗学》2007年第7期804-807,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:探讨马齿苋水提液保护衰老小鼠DNA端粒长度缩短的作用机制。方法:采用小鼠注射D-半乳糖制成衰老小鼠模型,随机将模型小鼠分为高、中、低3个浓度组和模型组,另设正常对照组。高、中、低浓度组在标准饲料基础上,每日每只分别灌喂2.5、5.0、10.0g/mL马齿苋水提液0.4mL,饲喂4周。聚合酶链反应-免疫酶联吸附试验法检测衰老小鼠脑组织端粒酶活性,端粒限制性片段Southernblot法检测脑组织端粒长度变化。荧光实时定量PCR法检测脑组织c-myc和p53基因mRNA的表达,Western blotting检测脑组织C-MYC和P53的蛋白表达。结果:马齿苋水提液各组实验动物脑组织端粒酶活性明显升高,组织端粒长度的缩短得到有效保护。脑组织p53基因的表达下调,c-myc基因的表达不受影响。结论:马齿苋水提液抗衰老作用可能是通过抑制衰老小鼠脑组织P53基因表达,激活端粒酶的活性,从而延缓衰老小鼠DNA端粒长度的缩短。AIM： To investigate the protective effect of water extract of Purslane herb on telomere length in senile mouse. METHODS： The senile mouse models induced by D-gal were divided randomly into five groups： normal control group, 3 experimental groups and model group. Animals were fed with nonnal diet in control and model groups and with nonnal diet plus 2.5, 5.0, 10.0 g/mL water extract of Purslane herb respectively in experimental groups. The telomerase activation of senile mouse brain was detected by PCR-TRAP-ELISA. The lengths of telomerie restriction fragments （TRFs） were detected by southern-blot assay. The mRNA and protein expression levels of c-myc and p53 gene were detected by real-time fluorescence quantitative RT-PCR and western blot. RESULTS： The telomerase activity in senile mouse brain was significantly increased after treated with water extract of purslane herb. The telomere length in senile mouse brain was improved by water extract of purslane herb. The expression of p53 gene was decreased and c-myc gene was not activated. CONCLUSION： Water extract of purslane herb has possibly delayed the aging of senile mouse through inhibiting the p53 gene expression and activating the telomerase to protect the length of telomere in brain.

关 键 词：马齿苋水提液 端粒 端粒酶 C-MYC基因 P53基因 

分 类 号：R965.2[医药卫生—药理学]