水蔓菁组织培养及植株再生研究  

Tissue Culture and Plantlet Regeneration of Veronica linariifolia subsp. dilatata

作　　者：刘帆[1] 汤福义[2] 田孟良[1] 乔晓[1] 官宇[1] 

机构地区：[1]四川农业大学农学院,四川雅安625014 [2]四川农业大学林学园艺学院,四川雅安625014

出　　处：《四川农业大学学报》2007年第2期117-120,共4页Journal of Sichuan Agricultural University

基　　金：四川农业大学青年科技创新基金。

摘　　要：采用组织培养方法,设置不同激素配比的培养基对水蔓菁(Veronica linariifoliasubsp.dilatata)不同外植体叶片、茎尖及茎段进了行研究。结果表明MS+6-BA 2.0 mg/L+NAA 0.5 mg/L诱导愈伤组织效果最佳,其中以茎尖的诱导效果优于其他外植体;MS+6-BA 2.0 mg/L+NAA 0.05 mg/L诱导愈伤组织丛生芽的分化效果最佳;1/2 MS+IBA 0.5 mg/L诱导丛生芽的生根效果最佳。To establish a high efficiency plant regeneration system of Veronica linariifolia., callus, rosette buds, and roots were induced by different combinations of hormone from leaves, stem-tips and stems of Veronica linariifolia. The results showed that the medium of MS＋ 6 - BA 2.0 mg/L＋ NAA 0.5 mg/L was the best for callus induction, the medium of MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0.05 mg/L was the best for bud differentiation, and the medium of 1/2 MS＋ IBA 0.5 mg/L was the best e for rooting.

关 键 词：水蔓菁 组织培养 植株再生 

分 类 号：S682.3[农业科学—观赏园艺]