伏马菌素B1对人外周血单个核细胞表面HLA-Ⅰ分子表达的影响  

Effects of Fumonisin on HLA-Ⅰ expression on human peripheral blood mononuclear cells in vitro

作　　者：邢凌霄[1] 申海涛[1] 李月红[1] 王俊灵[1] 严霞[1] 王凤荣[1] 张祥宏[1] 

机构地区：[1]河北医科大学基础所病理室,河北石家庄050017

出　　处：《细胞与分子免疫学杂志》2007年第9期794-796,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：河北省自然科学基金资助项目(C2004000613);国家科技部基础研究重大项目前期研究专项资助项目(2001CCC00500)

摘　　要：目的:探讨伏马菌素B1(FB1)对体外培养的人外周血单个核细胞表面(HPBMc)HLA-Ⅰ分子表达的影响。方法:采用流式细胞术(FCM)、Western blot及半定量RT-PCR方法,研究不同浓度FB1(10和50μmol/L)处理后人外周血单个核细胞表面HLA-Ⅰ分子表达的变化。结果:FCM定量分析结果表明,经FB1处理24 h后,两组FB1处理细胞HLAⅠ-的平均荧光强度均较对照组降低(P<0.05),但是在两个处理组之间无统计学意义。Western blot也证实了上述结果。在mRNA水平上,分别检测了HLA-Ⅰ分子3个等位基因HLA-A,HLA-B,HLA-C的表达情况。结果显示,FB1处理后,HLA-A、HLA-B mRNA的表达均没有明显影响,仅HLA-C mRNA的表达较对照组降低。结论:10和50μmol/L FB1处理24 h可抑制人外周血单个核细胞表面HLA-Ⅰ分子的表达。AIM： To study the effects of Fumonisin B1 （FB1） on HLA-I on human peripheral blood mononuclear cells （PBMC） in vitro. METHODS： The expression of HLA-I on PBMC by FB1 pretreatment at different dosage（ 10, 50μmol/L） was detected with flow cytometry （FCM）, Western blot, and semi-RT-PCR, respectively. RESULTS： The expression of HLA- I on PBMC in vitro at the two experimental concentration was both lower than that of the control after FB1 treatment for 24 h as represented by fluorescence intensity by FCM analysis. Western blot results further confirmed the above results. At mRNA level, HLA-A, B and C mRNA were detected by RT-PCR, and the results showed that no changes were found on the expression of HLA-A, B mRNA between FB1 treated group and the control group, but HLA- C mRNA was inhibited in FB1 treated groups. CONCLUSION： l0 and 50 NmoVL FB1 could inhibit the expression of HLA- I on human PBMC in vitro at 24 h treatment.

关 键 词：伏马菌素B1 HLA-I分子 人外周血单个核细胞 

分 类 号：R392.11[医药卫生—免疫学]