RGDS四肽促进大鼠肝星状细胞胶原降解机制的研究  

The degrading mechanism of collagen promoted by RGDS in hepatic stellate cell

作　　者：刘丽[1] 姜慧卿[1] 张晓岚[1] 赵东强[1] 宋梅[1] 

机构地区：[1]河北医科大学第二医院消化科,河北石家庄050000

出　　处：《细胞与分子免疫学杂志》2007年第9期801-803,809,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：河北省科技攻关课题(01276134);河北省自然科学基金资助项目(301361)

摘　　要：目的:观察精-甘-天冬-丝氨酸(RGDS)四肽对纤维连接蛋白(FN)刺激的大鼠肝星状细胞(HSC)基质金属蛋白酶-13(MMP-13)及其组织抑制因子(TIMP-1)表达的影响。方法:应用体外细胞培养技术,用RT-PCR检测HSC MMP-13mRNA水平;原位杂交和Western blot技术分别检测上述细胞TIMP-1 mRNA和蛋白水平。结果:RGDS四肽干预2 h组MMP-13 mRNA的表达强度明显上调,同时TIMP-1 mRNA表达受抑制;RGDS四肽干预24 h组TIMP-1蛋白表达受抑制。结论:RGDS四肽诱导HSC MMP-13表达,抑制其TIMP-1表达是其抗肝纤维化的作用机制之一。AIM： To study the effect of Arg-Gly-Asp-Ser （RGDS） tetrapeptide on the expression of matrix metalloproteinase-13 （MMP-13） and the tissue inhibitor of metalloproteinase-1 （TIMP-1） in hepatic steUate cell（HSC） stimulated by fibronectin （FN）. METHODS： The expression of MMP-13 was evaluated by RT-PCR. TIMP-1 was analyzed by in-situ hybridization at mRNA level and by Western blot at protein level. RESULTS： Treated by RGDS tetrapeptide for 2 h, the mRNA expression of MMP-13 was upregulated but the expression of TIMP-1 mRNA and its protein in HSC was inhibited. CONCLUSION： The expression of MMP-13 can be induced by RGDS tetrapeptide and the expression of TIMP-1 can be inhibited by RGDS tetrapeptide, which is one of the mechanisms of its anti-fibrogenesis.

关 键 词：肝星状细胞 胶原 基质金属蛋白酶 基质金属蛋白酶组织抑制因子 精氨酸-甘氨酸-天冬氨酸-丝氨酸 

分 类 号：Q555.7[生物学—生物化学]