NF-κB p50亚基同源结构域相互作用多肽的筛选  

Screening of polypeptides interacting with Rel homology domain of NF-κB p50 subunit

作　　者：沈利群[1] 徐祥[1] 梁华平[1] 李生茂[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所一室

出　　处：《细胞与分子免疫学杂志》2007年第9期804-806,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家重点基础研究发展规划(973)资助项目(G1999054203);国家自然科学基金资助项目(30080009;30400569)

摘　　要：目的:应用酵母双杂交技术筛选获得与核因子κB(NF-κB)p50亚基同源结构域(RHD)相互作用的多肽。方法:应用酵母双杂交技术,以NF-κB p50 RHD为诱饵,筛选16肽cDNA文库,寻找与其相互作用的多肽,应用β-gal试验、酵母交配试验及一对一酵母回复性杂交等方法筛除假阳性克隆,确定阳性克隆。结果:经酵母双杂交及反复的排查,共获得8个阳性克隆。通过GenBank进行检索,未发现与之相匹配的蛋白序列。结论:获得8个与NF-κB p50 RHD相互作用的新型多肽,多肽的功能需进一步验证。AIM： To obtain the polypeptides interacting with NF-κB p50 Rel homology domain （RHD） by yeast two-hybrid technique. METHODS：Using NF-κB p50 RHD as bait, the polypeptides interacting with NF-κB p50 RHD were screened from a 16-pepUde cDNA library by yeast two-hybrid technique. The false positive clones were screened out but the positive clone were identifyied by β-GAL assay, yeast mating, and one to one yeast two-hybrid method. RESULTS： 8 positive clones were obtained. The gene sequences of the eight polypeptides were different from each other and their homologous proteins were not found in GenBank. CONCLUSION： 8 novel polypeptides interacting with NF-κB p50 RHD were obtained, but their functions need to be validated in further experiments.

关 键 词：核因子-ΚB 酵母双杂交系统 随机肽库 

分 类 号：Q781[生物学—分子生物学]