中国健康人外周血中具有CD4^+CD25^(nt/hi)CD127^(lo)特征的调节性T细胞频率  

Frequency of the CD4^+CD25^(nt/hi)CD127^(lo) regulatory T lymphocytes from the peripheral blood in Chinese healthy individuals

作　　者：王盈[1] 周磊明[1] 宫菊丽[1] 周明奎[1] 沈方伟[1] 郑晓虹[1] 张玮[1] 庄鸣华[1] 卢伟[1] 钟平[1] 潘启超[1] 康来仪[1] 

机构地区：[1]上海市疾病预防控制中心上海市艾滋病研究中心,上海200336

出　　处：《细胞与分子免疫学杂志》2007年第9期816-818,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：上海市医学重点学科建设项目资助(05Ⅲ029)

摘　　要：目的:初步确定健康人外周血中具有CD4+CD25nt/hiCD127lo特征的调节性T细胞(Treg)频率,为临床相关疾病的研究及Treg的分选提供参考。方法:采集312名8~60岁(5个年龄组)、不同性别健康人的静脉血,经三重免疫荧光染色,用流式细胞术分析CD4+CD25nt/hiCD127loTreg细胞频率,并观察细胞内Foxp3转录因子的表达。结果:健康人CD4+CD25nt/hiCD127loTreg细胞在外周血中约占CD4+T细胞的(6.55±0.11)%,各年龄组之间有差异(P=0.015),组内性别之间也存在统计学意义(P<0.05);CD25nt/hiCD127lo细胞特异性地表达Foxp3转录因子。结论:初步确定了中国健康人外周血中具有CD4+CD25nt/hiCD127lo表达特征的细胞频率,为Treg细胞的临床研究奠定了基础;CD25nt/hiCD127lo作为CD4+CD25+Treg细胞表面的特征性标志,可在分选时排除其他细胞干扰,获得较完整的Treg细胞。AIM： To determine the frequency of the CD4^＋ CD25^nt/hi CD127^lo regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals and provide some useful evidence for clinical research of correlative diseases. METHODS： From the CD4^＋ CD25^nt/hi CD127^lo regulatory T lymphocytes of peripheral blood in 312 Chinese healthy male and female individuals aged from 8 to 60 （five age groups were collected） The expression of transcription factor Foxp3 was detected by triplex immune fluorescence and the frequency of CD4^＋ CD25^nt/hi CD127^lo regulatory lymphocytes was determined by flow cytometry. RESULTS： The frequency of CD4^＋ CD25^nt/hi CD127^lo regulatory T lymphocytes in Chinese healthy individuals was （6.55 ± 0.11 ） %, and the frequency differed among age groups （ P = 0. 015 ） and sex groups（ P 〈 0.05 ）. CD4^＋ CD25^nt/hi CD127^lo regulatory T lymphocytes specifically express transcription factor Foxp3. CONCLUSION： The frequency of the CD4^＋ CD25^nt/hi CD127^lo regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals has benn preliminarily determined which lays the foundation for further clinical research of regulatory T lymphocytes. As a specific cell surface marker, CD25^nt/hi CD127^lo can helpful obtain pure CD4^＋ CD25^＋ regulatory T lymphocytes and suppress the interference of other cells duringcell separation.

关 键 词：中国健康人 CD4^+CD25^+调节性T细胞 CD127 FOXP3 

分 类 号：R392.11[医药卫生—免疫学]