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作 者:王盈[1] 周磊明[1] 宫菊丽[1] 周明奎[1] 沈方伟[1] 郑晓虹[1] 张玮[1] 庄鸣华[1] 卢伟[1] 钟平[1] 潘启超[1] 康来仪[1]
机构地区:[1]上海市疾病预防控制中心上海市艾滋病研究中心,上海200336
出 处:《细胞与分子免疫学杂志》2007年第9期816-818,共3页Chinese Journal of Cellular and Molecular Immunology
基 金:上海市医学重点学科建设项目资助(05Ⅲ029)
摘 要:目的:初步确定健康人外周血中具有CD4+CD25nt/hiCD127lo特征的调节性T细胞(Treg)频率,为临床相关疾病的研究及Treg的分选提供参考。方法:采集312名8~60岁(5个年龄组)、不同性别健康人的静脉血,经三重免疫荧光染色,用流式细胞术分析CD4+CD25nt/hiCD127loTreg细胞频率,并观察细胞内Foxp3转录因子的表达。结果:健康人CD4+CD25nt/hiCD127loTreg细胞在外周血中约占CD4+T细胞的(6.55±0.11)%,各年龄组之间有差异(P=0.015),组内性别之间也存在统计学意义(P<0.05);CD25nt/hiCD127lo细胞特异性地表达Foxp3转录因子。结论:初步确定了中国健康人外周血中具有CD4+CD25nt/hiCD127lo表达特征的细胞频率,为Treg细胞的临床研究奠定了基础;CD25nt/hiCD127lo作为CD4+CD25+Treg细胞表面的特征性标志,可在分选时排除其他细胞干扰,获得较完整的Treg细胞。AIM: To determine the frequency of the CD4^+ CD25^nt/hi CD127^lo regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals and provide some useful evidence for clinical research of correlative diseases. METHODS: From the CD4^+ CD25^nt/hi CD127^lo regulatory T lymphocytes of peripheral blood in 312 Chinese healthy male and female individuals aged from 8 to 60 (five age groups were collected) The expression of transcription factor Foxp3 was detected by triplex immune fluorescence and the frequency of CD4^+ CD25^nt/hi CD127^lo regulatory lymphocytes was determined by flow cytometry. RESULTS: The frequency of CD4^+ CD25^nt/hi CD127^lo regulatory T lymphocytes in Chinese healthy individuals was (6.55 ± 0.11 ) %, and the frequency differed among age groups ( P = 0. 015 ) and sex groups( P 〈 0.05 ). CD4^+ CD25^nt/hi CD127^lo regulatory T lymphocytes specifically express transcription factor Foxp3. CONCLUSION: The frequency of the CD4^+ CD25^nt/hi CD127^lo regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals has benn preliminarily determined which lays the foundation for further clinical research of regulatory T lymphocytes. As a specific cell surface marker, CD25^nt/hi CD127^lo can helpful obtain pure CD4^+ CD25^+ regulatory T lymphocytes and suppress the interference of other cells duringcell separation.
关 键 词:中国健康人 CD4^+CD25^+调节性T细胞 CD127 FOXP3
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